The DuraClone S34 Reagent Kit uses the principle of immunofluorescence to enumerate hematopoietic stem cells in cell preparations. It is available in a unitized, dried-down format and is ideal for enumerating viable CD34+ cells in cord blood samples on a flow cytometry platform. This reagent is intended for use on open flow cytometers. The DuraClone S34 Reagent Kit allows for flow cytometry based rapid and accurate assessment of live hematopoietic progenitor cell in accordance with the International Society of Hematotherapy and Graft Engineering (ISHAGE) guidelines. The success of hematopoietic transplantations and hematopoietic progenitor cell storage is relative to the absolute live CD34+ hematopoietic progenitor cells (HPC’s). The DuraClone S34 Reagent Kit facilitates a robust, single platform method of enumerating viable human CD34+ cells. The CD34 antigen is a 110 kDa single chain type1 trans-membrane glycoprotein, associated with human hematopoietic progenitor cell. The reagent contains workshop approved anti-mouse, anti-human monoclonal antibodies specific to the CD45 antigen, CD34 specific class III mouse anti-human monoclonal antibody, and 7-Amino Actinomycin-D (7-AAD) dye. Viability dye 7-AAD is a DNA intercalating agent that stains only cells in the late phases of cell death when the integrity of both the cell and nuclear membranes is lost. It is important to exclude CD34 dead cells to assist in counting true live CD34+ cells. Investigators have demonstrated that graft containing 4-5 X 106 CD34+ cells /kg recipient ideal body weight (IBW) will result in rapid, durable, tri-lineage engraftment.

Specifications

Specificity CD45, CD34
Form PE, Atto488
Clone H130|581
Flow Product Line DuraClone
Isotype IgG1 Mouse
Format Dry
Size 50 Tests
Regulatory Status CE
Target Species Human
UNSPSC 41116015

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The listed regulatory status for products correspond to one of the below:
IVD: In Vitro Diagnostic Products. These products are labeled "For In Vitro Diagnostic Use."
ASR: Analyte Specific Reagents. These reagents are labeled "Analyte Specific Reagents. Analytical and performance characteristics are not established."
CE: Products intended for in vitro diagnostic use and conforming to European Directive (98/79/EC). (Note: Devices may be CE marked to other directives than (98/79/EC)
RUO: Research Use Only. These products are labeled "For Research Use Only. Not for use in diagnostic procedures."
LUO: Laboratory Use Only. These products are labeled "For Laboratory Use Only."
No Regulatory Status: Non-Medical Device or non-regulated articles. Not for use in diagnostic or therapeutic procedures.