Allergenicity Kit, Cellular Analysis of Allergy consists of an optimized three-color combination of fluorescent monoclonal antibodies, an activation solution, a positive control for IgE-mediated basophil activation, a stop solution, a lysing solution and a fixative solution. The Allergenicity Kit is intended for the identification of resting and activated basophils based on an accurate basophils gating tool (utilizing CRTH2posCD203cposCD3neg). The analysis is performed on whole blood specimens. The cell population of interest is stained with monoclonal antibodies in the presence of the allergen or controls. Erythrocytes are then lysed prior to flow cytometry analysis. Once T lymphocytes (CD3+ cells) are excluded, basophils are identified using CRTH2 and CD203c expression. Non-activated and resting basophils are CRTH2posCD203cdimCD3neg, whereas in vitro activated basophils are CRTH2posCD203cbrightCD3neg. The flow cytometer must be equipped to detect forward scatter, side scatter and three fluorescence channels allowing the analysis of FITC-, PE-, and PC7- conjugated antibodies.

Specifications

Specificity Allergenicity Kit
Size 100 Tests
Regulatory Status CE
Target Species Human
UNSPSC 41116015

NOT ALL PRODUCTS ARE AVAILABLE IN ALL COUNTRIES
PRODUCT AVAILABILITY AND REGULATORY STATUS DEPENDS ON COUNTRY REGISTRATION PER APPLICABLE REGULATIONS

The listed regulatory status for products correspond to one of the below:
IVD: In Vitro Diagnostic Products. These products are labeled "For In Vitro Diagnostic Use."
ASR: Analyte Specific Reagents. These reagents are labeled "Analyte Specific Reagents. Analytical and performance characteristics are not established."
CE: Products intended for in vitro diagnostic use and conforming to European Directive (98/79/EC). (Note: Devices may be CE marked to other directives than (98/79/EC)
RUO: Research Use Only. These products are labeled "For Research Use Only. Not for use in diagnostic procedures."
LUO: Laboratory Use Only. These products are labeled "For Laboratory Use Only."
No Regulatory Status: Non-Medical Device or non-regulated articles. Not for use in diagnostic or therapeutic procedures.