Automated Nucleic Acid Sample Preparation Methods

We offer a growing portfolio of Biomek-automated nucleic acid sample prep methods utilizing our own Agencourt reagent kits and other leading technology vendors. Each method is demonstrated to generate quality data using real-world samples by internal application scientists and customer laboratories.  

Review our methods list below and supporting application overview documents to find the reagent kits you’re currently working with in your lab or those you plan to evaluate in the future.

DEMONSTRATED METHOD
BIOMEK i7
HYBRID
BIOMEK i7/i5
MULTICHANNEL
BIOMEK i5
SPAN-8
BIOMEK FXP
HYBRID
BIOMEK FXP/NXP
MULTICHANNEL
BIOMEX NXP
SPAN-8
BIOMEK 4000
DNA AND RNA PURIFICATION AND CLEAN UP/​SIZE SELECTION/​QUANTITATION/​NORMALIZATION/​POOLING
Agilent TapeStation 2200 Plate Setup for analysis of DNA Fragments
AMPure XP - PCR Purifcation
CleanSEQ - Dye Terminator Removal
COVARIS DNA Shearing Plate Setup for E220 Focused Ultrasonicators
Kapa Biosystems Library Quantifcation Kit - Illumina (96 and 384)
qPCR/PCR Setup 384
qPCR/PCR Setup 96
Quantitation/Normalization
RNAClean XP - Post cDNA Purifcation and Post-IVT cRNA Purifcation
SPRIselect - Size Selection
Sample Pooling for Multiplexing
NUCLEIC ACID ISOLATION
CosMCPrep – High/Low Copy Plasmid Purifcation
DNAdvance - DNA Isolation from Tissue
FormaPure DNA - DNA Isolation from FFPE Tissue
FormaPure - RNA Isolation from FFPE Tissue
FormaPure Total – Total Nucleic Acid Isolation from FFPE Tissue
GenFind v2 - DNA Isolation from Blood
Mo Bio PowerMag Soil DNA Isolation Kit
RNAdvance Blood - RNA Isolation from Blood
RNAdvance Cell - RNA Isolation from Cells
RNAdvance Tissue - RNA Isolation from Tissue

Method Key

 
Demonstrated Method. This indicates that the method was developed for a sample preparation kit following a vendor’s published manual protocol. Each one is tested with scientifically relevant samples and has yielded results that meet the kit vendor’s specifications either in a customer lab (customer demonstrated) or in a Beckman Coulter Life Sciences Lab (Beckman demonstrated). Beckman Coulter makes no claims regarding the use or performance of these methods.
 
Illumina Qualified Methods, This indicates that Illumina’s analysis of libraries prepared with the Biomek-automated method has shown the libraries to perform comparably to those prepared manually. This method is not an Illumina product, and Illumina does not directly support this product. Illumina makes no representations or warranties with respect to this product.
 
Method created and provided by kit vendor.