Visualizing immune response
November 19, 2020
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Visualizing immune response: IOTest Myeloid Activation CD169-PE/HLA-DR-APC/CD64-PB Antibody Cocktail.*
Date: December 4, 2020
Time: 9:00am PST, 12:00pm EST
Activation of Myeloid cells and interferon release is an early response to infection. The 3-marker combination (CD169/CD64/HLA-DR) gives an excellent overview of the innate immune response. The flow cytometry assay can effectively differentiate the immune response to bacteria or viruses.
Bacterial stimuli: CD64 overexpression on neutrophils (nCD64).
Viral stimuli: CD169 overexpression on monocytes (mCD169).
Recent stimulus: increased HLA-DR, Overstimulation/Immune exhaustion: decreased HLA-DR.
Further, the assay, using whole blood, is rapid and easy to perform, making this an invaluable tool in clinical research on antibiotic resistance, and studying interferon signaling during infections.
Describe Myeloid cell activation and how it is specific for viral and bacterial infections due to differential interferon signaling.
Explain how Markers CD64 and CD169 can be effectively used to differentiate between the types of infections.
Shankar Pattabhiraman, Ph.D.