AUC Symposium - Multiple Wavelength Analysis of Proteins
February 25, 2021
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Please join us for our second AUC Symposium covering Multi-Wavelength Analysis of Proteins
Date: Thursday February 25, 2021
Time: 1:00pm EST
Studying Macromolecules in Mixtures using Mechanical Marvels
Abstract: The most pressing questions in basic research revolve around the behavior of biological macromolecules in their native milieu. Many times, these macromolecules are assembled within a larger macromolecular assembly that is a composite of not only protein, but also nucleic acid, carbohydrate, or lipid.
In this session, we will review how AUC can accelerate basic research and enhance the information gathered from complementary structural and biophysical techniques, including X-ray crystallography and cryo-EM, light scattering, and small-angle X-ray and neutron scattering (SAXS/SANS).
Using Sedimentation Equilibrium to study the stoichiometry and binding affinity of macromolecular complexes.
Abstract: Sedimentation Equilibrium Analytical Ultracentrifugation (SE-AUC) is a powerful technique for studying protein-protein interactions in solution. In this presentation, I will show how SE-AUC was used to study the oligomeric state of a self-associating protein and to also resolve the stoichiometry of macromolecular hetero-complex. The considerations for experimental design will also be discussed.
|Kushol Gupta, Ph.D.||Matthew Ranaghan Ph.D.|
|Research Assistant Professor||Research Scientist|
|Department of Biochemistry and Biophysics at the University of Pennsylvania||Center for the Development of Therapeutics (CDoT) at the Broad Institute of MIT and Harvard|