The CytoFLEX S (Blue, Red, Violet, Infrared Series) has the capacity for 15 parameters, including 13 for fluorescence detection. The fully activated instrument includes four channels from the 488 nm (Blue) laser, three from the 638 nm (Red) laser, four from the 405 nm (Violet) laser and two from the 808 nm (Infrared) laser. Instruments with as few as four fluorescent channels activated are available with the ability to activate additional parameters as needed by purchasing an activation key.

  • Infrared laser provides untouched fluorescent channels with minimal spectral overlap into traditional channels
  • Fixed integrated optics and quartz flow cell design enables portability
  • Avalanche Photodiode (APD) detectors provide unmatched red sensitivity and unique compensation workflow
  • Violet Side scatter (VSSC) optional configuration for nanoparticle resolution


Sample handling

  • Semi-automatic Single Tube Loading with 12 x 75 mm or 1.2 mL tubes
  • Optional plate loader for 96-well plates
  • Volumetric measurements with continuous flow
  • Fluidics provide continuous flow and volumetric measurements integrated into compact footprint


  • Novel Compensation Library: store fluorescent spillover values of dyes to easily determine the correct compensation matrix with new gain settings
  • Absolute linear gain amplification enables the use of compensation settings between experiments and sample types

Product Specifications

Detection Photodiodes
Resolution <300 nm particles (488 nm side scatter), <200 nm particles (405 nm side scatter); rCV 3%
Performance <30 MESF for FITC, <10 MESF for PE
Optics/Laser Power 488 nm solid state laser (50 mW), 638 nm solid state laser (50 mW), 405 nm solid state laser (80 mW), 561 nm solid state laser (30 mW)
Regulatory Status RUO
Flow Cell Patent-pending, alignment-free integrated optics quartz flow cell (420 μm x 180 μm ID) design with >1.3 numerical aperture
Signal Processing Digital system with 7-decade display
Power Requirements 100-240 VAC, 50/60 Hz
Item Specifications Referenced B75408