Performance testing of cytometry instruments is utilized to characterize the capability of the cytometer to perform high complexity applications. We have tested the new CytoFLEX flow cytometer against the performance measurements for sensitivity, dynamic range, population resolution, linearity, stability, reproducibility, and small particle resolution. Commercially available bead standards were used to evaluate performance expectations across multiple parameters.
The CytoFLEX flow cytometer was evaluated at La Jolla Bioengineering Institute, Purdue University Cytometry Laboratory (PUCL) and the Beckman Coulter, Miami site. Additional data was collected through a collaborative effort with Al Donnenberg from University of Pittsburgh Cancer Center and School of Medicine at Sun Yat-Sen University, Guangzhou, China. Each instrument was equipped with three lasers (405nm, 488nm and 638nm) and 12 parameters with 9 fluorescence detectors. Instruments were equipped with the enhanced Violet laser Side Scatter Channel (VSSC) option for small particle detection.