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Automated Base Calling |
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Sequence analysis includes signal processing, base calling and the
determination of confidence values for each
called base. No calibrations are required to compensate for spectral
overlap or mobility variations between the dyes. A two pass base-caller
first identifies significant peaks then employs a directed graph
technique* to refine the called base sequence. Finally, the error-probabilities
of called bases are estimated and quality values are assigned. Automated heterozygote detection
can also be selected allowing the user to
identify heterozygous base positions in genomic DNA.
(Click the image to see a full-size version of the graph)
*Berno, A. 1996. A graph theoretic approach to the analysis of DNA
sequencing data.
Genome Res. 6:80-91 .
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