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T3 Update On-Line--November 2004 |
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In this third on-line issue of T3 Update we provide
Beckman Coulter users of Automated Solutions with Tips, Tricks and
Techniques for Academic and Drug Discovery Research.

From the Editor: "Verified vs. Validated Solutions"
The automated methods we provide for our customers, scientists in Academia, Small Biotech and Large BioPharma, have been developed to two different levels of development: Verified and Validated Solutions.
more

Application Information
- Using Beckman Coulter's Biomek 3000 and Biomek NX Laboratory Automation
Workstation for the Purification of High-Quality Total RNA, by
Ruth Zhang, Chad Pittman, Laura Pajak and Graham Threadgill, Beckman Coulter, Inc.
more

- A Novel System for Automated RNA Isolation: Increasing Throughput Without Increasing
Footprint, by Bryan Laffitte, David Murray, Ginger Smith, Jim Liacos,
Amy Siu, Cathy Finlay, and Jimmy Bruner, GlaxoSmithKline, Research Triangle Park, NC.
more

- Automated Purification of His-Tagged Proteins in a 96-well Format, by
Zhu Zhu, Keith Roby and Graham Threadgill, Beckman Coulter, Inc.
more

- Automation Method for Protein Purification using Promega's MagneHis*
Protein Purification System, by Hiromasa Kashimori,
Beckman Coulter K.K., Japan.
more

- Automating Promega Cell-Based Assays in Multiwell Formats,
by Tracy Worzella and Brad Larson, Promega Corp., Madison, WI.
more

- Abundant-Protein Depletion Coupled with Multidimensional Fractionation
of the Human Plasma Proteome, by Edna Betgovargez and Michael Simonian,
Beckman Coulter, Inc.; Lei Huang, Jerald S. Feitelson, and Xiangming Fang,
GenWay Biotech, Inc., San Diego, CA. more


Assay Workstation
- New Workstation Delivers Complete Solution for ELISAs and Cell-Based
Assays. Press Release more

- An Automated ELISA to Determine Interleukin-8 Protein Expression in a
Human Lung Carcinoma Cell Line, by Chad Pittman, Laura Pajak, Ruth
Zhang and Graham Threadgill, Beckman Coulter, Inc.
more

- Automation of a Cell-Based Screening Assay for Intracellular
Apoptosis Activity using the Biomek Assay Workstation, by Yu Suen,
Keith Roby, Javorka Gunic and Graham Threadgill, Beckman Coulter, Inc. more


Integrated Solutions
- A Custom Serial Dilution Step has been written for the Biomek
FX to utilize a 96- or 384-channel head for serial dilution across a single plate.
more

- Real Time Data Display is an application that captures SILAS™
data messages from a microplate reader and displays the results in a graphical format.
more

- The Biomek FX Microplate Flattening ALP is a custom product
for flattening PCR* microtiter plates on the Biomek FX for more reliable pipetting.
more

- The Biomek FX Plate Rotating ALP is a custom product
designed for horizontal rotating of microtiter plates about the
center of the plate from standard "landscape" orientation to a
"portrait" orientation and back on the Biomek FX.
more

- The Biomek FX 16–Position Heating/Cooling ALP is used
to provide 16 temperature-controlled labware positions on the
deck of the Biomek FX. more


Project Management – North America Operations (NAO)
- Biomek FX Assay Workstation solution for sample prep and ELISA Assay Automation. An automated solution was needed for the dilution of the rodent sera, distribution in 24- and 96-well plate formats and performance of ELISA assays to monitor for an immune response.
more

- Biomek FX Assay Workstation solution for running multiple ELISA assays.
An upgrade from a Biomek 2000 Core system to a Biomek FX Assay Workstation provided increased capacity for the processing of multiple ELISA assays.
more

- Biomek FX Assay Workstation solution for ELISA assays in a BioProtect Hood. An
automated solution for the processing of cells (cell feeding), performance of ELISA assays
and confirmation of the results from positive wells in secondary ELISA assays.
more

- Biomek FX-based Core System Solution for Cell Plating, Transfection and Screening. An automated solution for cell plating, performing transfection reactions and screening the resultant cellular clones that was designed, installed and fully functional in less than six months.
more


Customer Training and Support
Automation Training -- A Letter of Thanks: A letter from Jennifer Wiseman (Senior Lab Technician, UK-Advanced Genetic Technologies Center (AGTC), University of Kentucky) for training received in the Biomek FX Advanced Methods course.
more

Frequently Asked Questions
The Call Center: We provide a world-class customer support team to guide you
-- from consultation in choosing products and services, through installation to training and support
-- to help you end up with a successful solution-based package. more

Training and Exhibitions
North America more
Europe more
Japan and Far East more
Beckman Coulter Events Calendar more

For comments or questions about T3 Update, please contact
David Daniels, Ph.D., editor.
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