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T³ Update On-Line--June 2004

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In this second on-line issue of T³ Update we provide Beckman Coulter users of Automated Solutions with Tips, Tricks and Techniques for Academic and Drug Discovery Research.

From the Editor: Why Automate?

I confess that I take it for granted that all scientists in academia, small biotech and large biopharma doing biological research have a clear understanding of what automation has to offer them in their daily routine. That said, I am sometimes caught by surprise when I’m asked this question. My first response is that automation allows you to do more, but throughput is not the only reason to automate! more

Application Information

High-Throughput Real-Time PCR* Reaction Preparation: Setup of Automated Protocols Using the Biomek 2000 Laboratory Automation Workstation, by Elena Barbaria, Grazia Saturno, Daniela Radice, Pharmacia Italia. more
Automatic In-Gel Protein Digestion and MS Sample Preparation, by Antonino Biroccio, Chiara Aceti, Andrea Urbani and Giorgio Federici. more
Automatic Protein Purification: Automated Polyhistidine-Tagged Protein Purification Using the MagneHis* Protein Purification System, by Michael Bjerke, Laurie Orr, Cristopher Cowan, Becky Godat, Promega Corporation. more
An LC-MS/MS-Based High-Throughput Screening Method for Cytochrome P450 Inhibition Assay in a 384-Well Plate Format, by Mingdan Wu, Robert Singleton, Deepthi Reddy, Vinolia Chellaraj, Rehan Malik, Manish Soni, Roy Vaz Aventis Pharmaceuticals, Bridgewater, NJ. more

Integrated Solutions

Integration of Variomag Thermoshake on the Biomek FX
The Variomag Thermoshake, a microplate temperature-controlled plate shaker sold by Inheco, will be integrated by Beckman Coulter, Inc. onto a Biomek FX Laboratory Automation Workstation, using SILAS™ integration technology. more
Biomek 2000 Specification for Integration of Hit Picking Wizard Software
A Hit Picking Wizard software utility has been developed for the Biomek 2000 Laboratory Automation Workstation. more
Custom Hit Picking Using Biomek FX Wizard for Span-8
Biomek FX Hit Picking Wizard for Span-8 only is a system designed to perform custom hit picking operations. more
Biomek 2000 Specification for Integration of Handheld Bar Code Reader
A Handheld Bar Code Reader will be used to read the bar code identification on vials to be placed on the liquid handler. more
Biomek FX Specification for Integration of Handheld Bar Code Reader
A Handheld Bar Code Reader will be used to read the bar code identification on vials to be placed on the liquid handler. more
SAGIAN™ Specification for Integration of Biomek FX Solvent Selection Valve
The Biomek FX Solvent Selection Valve is a custom product produced by Beckman Coulter to provide the ability to switch between six system fluids on a Biomek FX equipped with Span-8. more
Specification for Integration of Cell Scraper ALP
The Cell Scraper ALP is a custom product produced by Beckman Coulter for scraping a single well plate and tilting the plate for Span-8 aspiration. more
SAGIAN™ Core System Specifications for Integration of Applied Biosystems Prism* 7900HT Sequence Detection System
The Prism7900HT is a high-throughput real-time PCR system manufactured by Applied Biosystems. The Prism7900HT will be integrated by Beckman Coulter, Inc. into a SAGIAN Core System using SILAS™ integration technology. more

Project Management – North America Operations (NAO)

A customer who maintains an archival stock of various organism and DNA clones in bacterial culture has the challenge of making these samples available to researchers worldwide. To support this business, the Project Management Team in NAO provided a solution that enabled this customer the ability to execute unattended hit picking jobs consisting of hundreds of source plates and large-scale plate replication tasks, including barcode labeling. more

Customer Training and Support

Beckman Coulter Automation Training

Beckman Coulter offers several courses in North America to help you learn to use your Biomek liquid handlers and Integrated Systems. Biomek classroom courses are offered in three Beckman Coulter training facilities: Fullerton, Calif., Hoffman Estates, Ill., and Somerset, N.J. If you cannot attend a two-day course but need to learn how to use variables, work lists, or the Span-8’s Transfer from File function, eLab Live On-line can provide the classroom quality with the convenience of the Internet. more

Frequently Asked Questions

SAMI® Log File Sorting: SAMI Tips and Tricks
Question: By default, all data generated on one reader during one SAMI run will be logged in one ASCII text file. If this SAMI run includes different assays using the same reader, sorting the log file can be tedious. Is it possible to create one log file per assay type for plates using the same reader device in a single SAMI method?
Answer: more

Horizontal Loading of Carousel and CO₂ Incubator: SAMI Tips and Tricks
Question: Is it possible to schedule a method, so that the plates in the CO₂ incubator are stored horizontally rather than vertically?
Answer: more

Training and Exhibitions

North America more

Europe more

Japan and Far East more

Beckman Coulter Events Calendar more

For comments or questions about T³ Update, please contact David Daniels, Ph.D., editor.