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T³ Update On-Line--March 2005

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In this fifth on-line issue of T³ Update we provide Beckman Coulter users of Automated Solutions with Tips, Tricks and Techniques for Academic and Drug Discovery Research.

From the Editor:

At LabAutomation 2005 we held a workshop titled Applications Automation on the Biomek family of Liquid Handlers: Partnerships that deliver solutions. This issue of T³ Update features much of the content of the workshop. If you'd like more information, please respond at the email address below. more

Application Information

Verified Solutions

Automated RNA Purification for Analysis of Interleukin-8 Gene Expression in a Pulmonary Epithelial Cell Line, by Ruth Zhang, Ph.D., Chad Pittman, Laura Pajak, Ph.D., and Graham Threadgill, Ph.D., Beckman Coulter, Inc. more
Automated Target Preparation of Universal Human Reference RNA on the ArrayPlex SA* using Enzo Amplification Kit, by Handy Yowanto and David Helphrey. Array Automation, LLC, Beckman Coulter, Inc. more
Automated Purification of His-tagged Proteins in a 96-well Format, by Zhu Zhu, Keith Roby and Graham Threadgill, Ph.D., Beckman Coulter, Inc. more
An Automated ELISA to Determine Interleukin-8 Protein Expression in Pulmonary Epithelial Cell Line, by Chad Pittman, Laura Pajak, Ph.D., Ruth Zhang, Ph.D., and Graham Threadgill, Ph.D., Beckman Coulter, Inc. more
Automation of a Cell-Based Screening Assay for Intracellular Apoptosis Activity using the Biomek® Assay Workstation, by Yu Suen, Ph.D., Keith Roby, Javorka Gunic and Graham Threadgill, Ph.D., Beckman Coulter, Inc. more

Validated Solutions

Automation of Double-Sink* PAMPA Permeability Assay on the Biomek FX Laboratory Automation Workstation, by Yu Suen, Ph.D., Konstantin Tsinman(1), Zhu Zhu, and Graham Threadgill, Ph.D., Beckman Coulter, Inc. and pION, Inc.(1) more

Automated Applications Workshop at Lab Automation 2005

Sigma-Aldrich Corp.:

A Novel Approach for Rapidly Extracting and Amplifying Genomic DNA for High-Throughput Genotyping Applications, by Danhui Wang, Sigma-Aldrich Corp. more
Extract-N-Amp* PCR* Kits: A Novel and High-Throughput Approach for Rapidly Extracting and Amplifying Genomic DNA from Animal Tissues, by Danhui Wang, Carol Kreader, Scott Weber, Derek Douglas, Jessica Copeland, Jennifer Van Dinther and Rafael Valdes-Camin, Sigma-Aldrich Corp. more
Automated Method for Integrated Lysis and Affinity Purification (iLAP*) of Histidine Containing Recombinant Proteins used for Protein Analysis, by Jonathan Stephan, Richard Mehigh, Danhui Wang, Jennifer Van Dinther and Rafael Valdes-Camin, Sigma-Aldrich Corp. more

Invitrogen Corp.:

High-Throughput ORFeome Cloning and Expression on the Biomek FX Liquid Handling System Using Invitrogen's Gateway* and Expressway*-HTP Technologies, by Noel Janney. Invitrogen Corp. (poster) more
High-Throughput ORFeome Cloning and Expression on the Biomek FX Liquid Handling System Using Invitrogen's Gateway* and Expressway*-HTP Technologies, by Noel Janney, Keith Roby(1), Ashley Getbehead, Yolanda Bell, David Daniels(1) and Jon Chesnut. Invitrogen Corporation and Beckman Coulter, Inc.(1) more

Millipore Corp.:

New Millipore Applications for the Biomek FX. 384-well Filter Based Enzyme Assays: Receptor Ligand Binding and Kinase Applications, by Marcy Engelstein, Millipore Corp. more
Advances in Vacuum Filtration, by Marcy Engelstein, Steve Sheridan and Libby Kellard, Millipore Corp. more

Promega Corp.:

Multiplexing Promega's Cell-Based Assays Using Beckman Coulter Instrumentation, by Tracy Worzella. Promega Corp. more
Multiplexing Promega's Cell-Based Assays Using Beckman Coulter Instrumentation, by Tracy Worzella, Brad Larson. Promega Corporation. (poster) more

Product Updates

Consumables:

Transfer 1 mL Volumes With a Biomek NX or FX Span-8 System Using Biomek P1000 Tips, by Bob Lund, Beckman Coulter, Inc. more
Pipette Valuable Samples From the Bottom of Deep Well Labware With Extended-Length Biomek P50 Tips, by Bob Lund, Beckman Coulter, Inc. more

Accessories:

Biomek NX: Ozgene bags an NX! by Jason Fawcett, Beckman Coulter, Inc. more

Software:

SAMI® Workstation EX "Biomek Evolved", by Mike Ashley, Beckman Coulter, Inc. more

Tradeshow Participation in 2005

Europe more

Japan and the Far East more

Beckman Coulter Events Calendar more

* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffmann-LaRoche, Ltd.

For comments or questions about T³ Update, please contact David Daniels, Ph.D., editor.