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BiomekŪ FX Specification for Integration of Serial Dilution Step
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Overview
This Custom Serial Dilution Step has been written for the BiomekŪ FX to
utilize a 96- or 384-Channel head for serial dilution across a single plate. The
Multichannel head will be loaded with one column of tips along the left side
(Column 1) of the head, or with one row of tips along the backside (Row 1) of
the head. The rest of the head will be loaded with shorter tips*. The longer
tips will be utilized for the serial dilution process.
The warranty for this integration is one year from shipment. This warranty
applies for the integration to the version of software originally integrated.
Any changes to system components that cause the integration to no longer
function properly are not covered under this warranty.
The warranty applies to the system and system software versions on which the
original integration was installed. Although Beckman Coulter's goal is for the
integration to be forward compatible, this cannot be guaranteed for custom
integrations. Any upgrades to the system that cause the integration to no longer
function properly are not covered under the warranty for this integration.
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Functional Description
This integration allows the customer to perform serial dilutions across a
single 96- or 384-Well plate on a Biomek FX utilizing a Multichannel head. The
serial dilution can be performed across the rows or columns of a plate. If using
a 96-channel head, AP96 200 tips must be loaded on row 1 or column A*. All other
tips must be AP96 20 tips. With a 384-channel head, AP384 30 tips must be loaded
on row 1 or column A. All other tips must be tips that have been cut off. In
order to load tips on the Biomek FX, a full tip box must be used*.
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A
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B
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| Figure 1. Use of the custom Biomek FX Modified Receivers allows for the loading and unloading of a single row or column of tips from a standard Biomek FX tip box for use with a 96-channel head, (A) or the 384-channel head, (B) in this serial dilution method. |
The following variables may be set for the serial dilution:
- Pod to run the serial dilution step
- Tip type with option of unloading or keeping tips on after transfer
- Wash tip option with number of cycles per wash; washing after transferring
diluent; washing after transferring source compound from master plate, and
washing when finished with serial dilution step.
- Plate position to perform dilutions
- Dilution direction (left to right or top to bottom)
- Start and end (row or column) of dilution
- Volume and liquid type selection
- Technique selection for both the dilution, diluent, and source compound
(similar to Biomek FX aspirate and dispense techniques)
- Discard excess volume and deck position of dilution
- Abilities to add diluent before transfer, and to the left-most column
- Diluent position on Biomek FX deck
- Liquid type of diluent
- Labware type in which holds diluent**
- Dilution ratio
- Master plate position
- Labware of master plate
- Overriding auto-selected technique can also set other variables such as
tip touch, blowout, mix count, speed and volume.
This integration will support serial dilutions across 96- or 384-well plate
types supported by the Biomek FX software.
Device Control
All tasks are controlled through the Biomek FX software. The Multichannel pod
serial dilution step can run as a single or multiple steps within a Biomek FX
method. No additional hardware or software is required*.
Data Collection
There are no data files generated from the process, other than a Biomek FX
standard log file showing general tasks performed during the method operation.
Additional Specifications
| Plate Types |
Any standard 96- or 384-well plates supported by the
Biomek FX software |
Integration Components
The integration package for this system includes the following items:
- Custom Biomek FX Multichannel pod serial dilution step
- Installation and User's Guide
* The modified tip arrangements described within these specifications are not
necessary if the user purchased the custom Biomek FX Modified Receivers that
allow the loading and unloading of single row or column of tips from a standard
Biomek FX tip box.
** If aspirating from a reservoir, liquid level tracking may not be accurate
due to the fact that the Biomek FX software operates as if it is aspirating or
dispensing a full array of tips on the multichannel head. Therefore, if
aspirating, the tips may lower faster in the liquid; if dispensing, the tips
will rise faster than the liquid.
For comments or questions about T3 Update, please contact
David Daniels, Ph.D., editor.
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