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A customer came to Beckman Coulter to obtain an automated solution for the processing of cells (cell feeding), performance of ELISA assays and confirmation of the results from positive wells in secondary ELISA assays. This customer was provided an update to SAMIŪ 1.5 / Biomek FX software to enable cherry picking and sample tracking. The project management team designed a Biomek FX Assay Workstation in a Baker BioPROtect* II clean air and containment enclosure to satisfy the automation needs of the customer. At the center of the Assay Workstation was the Biomek FX with a dual-bridge hybrid set up providing both the Span-8 and a 96-Multichannel head. Components integrated on the deck of the Biomek FX included an ELx405* washer (Bio-Tek Instruments, Inc.) and a SpectraMax* microplate reader (Molecular Devices). A Cytomat Microplate Hotel, connected to the deck of the Biomek FX via a conveyor ALP, was also enclosed within the BioPROtect II enclosure.
The key to success for the customer was solving their cherry picking and sample tracking needs. The customer was not yet using barcodes in their process, so samples were tracked based on their Transport Name. This information was sent to the Biomek FX by use of a variable (barcode) in the FX module where its value was mapped to the Transport Name. The same variable (barcode) is defined in the FX method. A new value (Transport Name) of the variable is transferred to the FX with each iteration of a Family in SAMI. For example, the cell plate in Family 1 would have the Transport Name of Plate_1. Plate_1 becomes the value of the barcode variable in both SAMI and the FX method. The Transport Name in Family 2 would be Plate_2. The customer was enabled to input the names for the plates in the SAMI Go screen either manually or via a file input. The barcode variable was used in the FX method to set the name of the plate, create the Reader Dataset and to name the Dataset Report file. The Report files were used in the cherry picking methods as input for the dataset creation and dataset processing steps to drive the cherry picking job. These were all run through SAMI where the destination plates reside on the deck of the FX and the source plates are fed through SAMI. Each Family represents a new source plate. The FX Marks and SAMI Labware Persistence were used to determine the correct destination well and plate to dispense the sample. The components used in this automated solution were chosen based on functionality, space and price. The customer is very happy with the automated solution that was developed by the team. * All trademarks are the property of their respective owners. For Research Use Only; not for use in diagnostic procedures. For comments or questions about T3 Update, please contact David Daniels, Ph.D., editor.
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