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GenomeLab™ Gene Expression Suite Automation: Reaction Setup and Plate Preparation using the Biomek® 3000 Laboratory Automation Workstation from Beckman Coulter

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Mary Blair, Kelly Marshall M.S, and Laura Pajak Ph.D., Beckman Coulter, Inc.

Figure 1. GenomeLab GeXP Genetic Analysis System Quantitative Gene Expression

The Gene Expression Suite of Methods for the Biomek 3000 workstation is designed to quantitate and normalize extracted RNA, prepare a reaction plate for RT-PCR* and PCR, and prepare a sample plate for the GenomeLab GeXP Genetic Analysis System. The methods are designed to simplify processing of samples for the GeXP system and can be customized for non gene expression studies.

This poster will focus on the last two methods in the suite: Reaction Setup and Plate Preparation. The Reaction Setup User Interface includes default buttons that allow the user to set up a RT-PCR or PCR plate using the parameters defined by the GeXP kit protocols. The customizable user interface contains options for the samples, master mix, and primers that enable Reaction Setup to double as a general purpose reaction plate setup utility outside of the GeXP system.

If a thermocycler is integrated on the workstation deck, the method can move the plate to the thermocycler and execute the amplification protocol providing hands-free processing. The Plate Preparation method automates the process of creating a buffer plate, dilution plate, and sample plate for processing by the GeXP Genetic Analysis System. Options such as the dilution factor are easily customized via the user interface, allowing the user to optimize each sample set.

* The PCR process is covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffman La Roche, Ltd.

Click here for this poster (PDF)

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Additional Information

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Application System Requirements

Hardware Options

Source	Part No.	Quantity	Description
Beckman Coulter, Inc.	986120	1	Biomek 3000 Laboratory Automation Workstation
Beckman Coulter, Inc.	A26572	1	GeXP Genetic Analysis System

Software Options

Source	Part No.	Quantity	Description
Beckman Coulter, Inc.	719349	1	Biomek Software

Consumables and Reagents

Source	Part No.	Quantity	Description
Beckman Coulter, Inc.	A21019	1	GenomeLab GeXP Start Kit
Beckman Coulter, Inc.	608012	1	Plate, Microtiter, Sample, V-bottom thermal, cycler-compatible, polypropylene, 200 ÂµL nomi
Beckman Coulter, Inc.	717253	1	Biomek AP96 P250 Pipette Tips, prestirile with barrier
Beckman Coulter, Inc.	609801	1	Plate, Microtiter, Sample, V-bottom thermal, cycler-compatible, polypropylene, 200 µL nomi
Beckman Coulter, Inc.	373661		24-Position Tube Rack
Beckman Coulter, Inc.	373696	1	Insert, Tube, 11 mm, White, for 1.5 mL Microfuge® Tubes (case of 25)
Beckman Coulter, Inc.	356090	1	Tube, Polypropylene, Snap-On Cap, Natural, 1.5 mL, 11 x 38 mm, 0.44 x 1.52 in (pkg of 500)
Beckman Coulter, Inc.	717252	1	Tip, P250, Biomek® AP96, Sterile, 240/220 µL (case of 10 racks)
Beckman Coulter, Inc.	717256	1	Tip, Barrier, P20, BiomekÂ® AP96, Sterile, 20 ÂµL (case of 10 racks)
Beckman Coulter, Inc.	609801	1	Plate, Microtiter, Sample, V-bottom thermal, cycler-compatible, polypropylene, 200 µL nomi
Beckman Coulter, Inc.	372790	1	Quarter Reservoir (case of 40)
Beckman Coulter, Inc.	372795	1	Frame for Reservoirs (1 Each)
Beckman Coulter, Inc.	609844	1	96-Well Microplate without Lid, Nonsterile (case of 100)

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* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffmann-LaRoche, Ltd.

For Research Use Only; not for use in diagnostic procedures.

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