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Generating Amplification Reactions Using the BioRAPTR FRD™ Microfluidic Workstation

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Amy Yoder, Laura Pajak, Ph.D. Beckman Coulter, Inc.

Figure 1: BioRAPTR FRD Microfluidic Workstation

Low-volume amplification reactions are desirable as a means to deliver high throughput and cost savings. This approach is challenging as it requires accurate dispensing of low-volume sample and reagents into a 384-well reaction plate. Beckman Coulter has automated this process by developing a protocol to dispense master mix using our new BioRAPTR FRD Microfluidic Workstation. The BioRAPTR FRD workstation provides a platform that produces highly accurate and precise dispensing while using volumes that range between 100 nL to 60 μL. This allows for a quick and easy setup of a reaction plate that can contain four to eight different master mixes depending on whether a 4-tip or 8-tip head is being utilized. This protocol was used to generate low-volume standard end-point PCR* reactions.

To further test the robustness of the method, STR analysis of human genomic DNA was performed using the GenomeLab™ STR Human Primer kit and CEQ™ 8800 Genetic Analysis System. Genomic DNA was dispensed into a 384-well plate. Master mix was added to the plate using the BioRAPTR FRD workstation. After amplification, the CEQ 8800 Genetic Analysis System was used for separation and fragment analysis.

This poster will describe the process flow for generating low-volume amplification reactions for STR analysis and the data obtained from samples processed using these protocols.

Click here for this poster (PDF)

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Additional Information

BioRAPTR FRD

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Application System Requirements

Hardware Options

Source	Part No.	Quantity	Description
Beckman Coulter, Inc.	B19294	1	Automated BioRAPTR FRD™ Workstation with 4-tip Head for 384-well plates
Beckman Coulter, Inc.	285595	1	CEQ™ 8800 Genetic Analysis System for Sequencing and Fragment Analysis, 110/230 V, 60/50 H

Consumables and Reagents

Source	Part No.	Quantity	Description
Beckman Coulter, Inc.	356090	1	Polypropylene Tubes with Snap-On Caps, 1.5 mL

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* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffmann-LaRoche, Ltd.

For Research Use Only; not for use in diagnostic procedures.

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