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Overview The Prism* 7900 HT is a high-throughput real-time PCR* system manufactured by Applied Biosystems. The Prism 7900 HT will be integrated by Beckman Coulter, Inc. into a SAGIAN™ Core System using SILAS™ integration technology. The warranty for this Custom Integration is one year from shipment. This includes Beckman Coulter-supplied hardware and software. This warranty applies for the integration to the version of third-party hardware, firmware, and software originally integrated. Any changes to the third-party component that cause the integration to no longer function properly are not covered under this warranty. The warranty applies to the system and system software versions (including SAMI®) on which the original integration was installed. Although our goal is for all SILAS modules to be forward compatible, this cannot be guaranteed for custom integrations. Any upgrades to the system (including SAMI) that cause the integration to no longer function properly are not covered under the warranty for this integration. Functional Description The Prism 7900 HT Sequence Detection System is designed to use 96-well and 384 well PCR plate formats. Only 384- well plates will be supported in this integration. The Applied Biosystems recommended plate is their ABI PRISM 384-Well Clear Optical Reaction Plate. Plates must be sealed prior to use in the Prism 7900 HT. Applied Biosystems recommends their ABI PRISM Optical Adhesive Covers. When using an automated sealer, seal material must be comparable to the ABI PRISM seal to achieve accurate results. Microplates can be transferred to and from the robot accessible microplate carrier. The ORCA® robot will transfer a plate to the device before a read is performed, then remove the plate after the read has completed. Device Control The Prism 7900 HT can be operated in two modes. The first mode of operation is through the control software that resides on the Prism 7900 HT computer. The device manufacturer supplies the Sequence Detection Software. The second mode of operation is through the SILAS/SAMI module for the Prism 7900 HT. The second mode is outlined below: In the SILAS/SAMI System the device is controlled through the Prism 7900 HT SILAS module**. In this implementation an operator will have options to initialize the device to establish a communications link through the TCP/IP connection, Open and Close the robot accessible microplate tray, and to Run a protocol that has been predefined by the operator. The user will be able to retrieve the predefined list of protocols from the Prism 7900 HT computer. The list of protocols can be refreshed if the user creates, edits or removes any of the protocols on the Prism 7900 HT computer. Once configured for the appropriate read program, the SAMI software is used to schedule the robotic run allowing the robot to load and unload microplates from the unit and perform the selected read functions. Any internal errors in the function of the Prism 7900 HT are communicated back to the host computer and displayed by an error notification box. Errors received from the Prism 7900 HT are considered fatal and will interrupt a SAMI run. All device errors are logged according to Core System standards. **Note that SILAS software must be present on the Windows* NT 4.0 based PC that will be used to run the instrument. SILAS and a Windows NT 4.0 based PC may be purchased as separate items, if needed. Data Collection All data will be stored in the custom ABATS database though data will be generated in the default SAMI Data Log. Additional Specifications
***Note that all performance and power specifications are those provided by the instrument manufacturer. Any discrepancies that arise with these specifications will be handled in accordance with the instrument manufacturer's warranty or replacement policy. Integration Components The integration package for the ABI 7900 includes the following items:
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffmann-LaRoche, Ltd. For comments or questions about T3 Update, please contact David Daniels, Ph.D., editor.
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