|
|
|||
|
|
||
|
|||
|
Overview A Hit Picking Wizard software utility will be developed for the Biomek 2000 Laboratory Automation Workstation. The wizard will allow the operator to specify how hits in row/column format are transferred to a destination plate. A worklist input file will specify the hit wells of the source plates. The Hit Picking Wizard will generate a BioWorks™ method based on the worklist input file and operator specified information. The operator can then run this method. A Destination Plate Map file and an Event Log file will be generated at run time. The Destination Plate Map file will list the well location and source plate ID for each well on the destination plate. The Event Log file will contain a list of bar codes of the source and destination plates processed by the system. The Hit Picking Wizard software for the Biomek 2000 will be created by Beckman Coulter. The wizard will consist of a series of screens in which user input will be taken. Within the wizard, the operator will select between Reformatting or Hit Picking. For Reformatting, the source pattern and destination pattern are defined for the transfer. For Hit Picking, a worklist input file will be used, specifying a list of wells in bar coded source plates to be transferred to destination plate(s). After collecting input, the wizard will create a Biomek 2000 method. This method will contain the steps necessary to move the source and destination plates from the stacker carousel to the deck of the Biomek 2000 (if selected), transfer the hits from the source to the destination (or perform reformatting), and return the plates to the stacker. An event log will be kept and a file listing the contents of the destination plates will be produced. In the case were no stacker carousels are used, all plates and tips will start on the deck. The warranty for this Custom Integration is one year from shipment. This includes Beckman Coulter- supplied hardware and software. This warranty applies for the integration to the version of third-party hardware, firmware, and software originally integrated. Any changes to the third-party component that cause the integration to no longer function properly are not covered under this warranty. The warranty applies to the system and system software versions on which the original integration was installed. Although our goal is for this software to be forward compatible, this can not be guaranteed for custom integrations. Any upgrades to the system that cause the integration to no longer function properly are not covered under the warranty for this integration. Functional Description The operator will provide a worklist file for the run. The worklist file will contain the plate number, plate barcode, hit well location in row/column format, volume to be transferred (optional), and well content ID (optional). This file will be specified in the Hit Picking Wizard interface. The operator will place on the deck, or load in the Stacker Carousel the plates specified in the worklist file in the exact order that they are in within the worklist file. The operator will start the Hit Picking Wizard and specify the worklist file to be used for the run. The following options will be supported:
Note: Reloading of the Stacker Carousel(s) during the middle of a BioWorks run cannot be utilized at this time. Only one Stacker Carousel load at the beginning of the method run, with included empty stacks for returning plates (or tip boxes), can be supported. If more source or destination plates are used that can fit in the Stacker Carousel(s), then an error message will be displayed requesting that fewer labware (a shorter run) be specified. Please edit the Worklist File to reduce the number of plates or tip boxes that are retrieved from the Stacker Carousel. This does not apply for a system without Stacker Carousels. This integration includes the Hit Picking Wizard software only as a custom option for the Biomek 2000 liquid handling system. Data Collection The Hit Picking Wizard will generate two data files. These files will be transferred prior to the run, or during the run as necessary. These files will be comma separated text files that can be used for importing into other utilities, such as Microsoft* Excel, for analysis. A Destination Plate Map file, and an Event Log file will be generated.
Additional Specifications
Integration Components The integration package for the Biomek 2000 Hit Picking Wizard software integration includes the following items:
* All trademarks are the property of their respective owners. For Research Use Only; not for use in diagnostic procedures. For comments or questions about T3 Update, please contact David Daniels, Ph.D., editor.
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
