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Currently, EDTA-stabilized whole blood is the most common sample type used for high-density genotyping. Blood has proven a very consistent and reliable source of genetic material for many avenues of testing and research, but it can also be a time-consuming, expensive and invasive collection method-- especially for long-term or broad range studies. Finding a comparable source of genetic material, such as saliva, that is more cost effective, more stable and less invasive would be extremely beneficial to the scientific community. This experiment used the Beckman Coulter Biomek® NXP Platform with Agencourt chemistry to extract genomic DNA from blood samples. DNA from the paired saliva samples were extracted using the manual extraction method provided by DNA Genotek. The study compared not only the DNA quality and quantity, but also the microarray call and concordance rates (CR) to indicate saliva’s suitability for genetic association studies. All samples were prepared and run simultaneously on the Genome-Wide SNP6.0 arrays (GW6.0)GeneChip microarrays consist of small DNA fragments (or probes), that are synthesized to specific locations on a coated arrays quartz surface. Millions of probes can be contained on one array. All the samples are scanned and analyzed using Affymetrix GeneChip Genotyping Console (AGGC). AGGC performs a multiple-chip analysis fitting probe effects to increase precision on signal estimates for the two alleles of each SNP, followed by a Bayesian classification approach to make genotype calls. Click here for this poster (PDF)
Additional Information:
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffmann-LaRoche, Ltd. For Research Use Only; not for use in diagnostic procedures. For comments or questions about T3 Update, please contact the T3 Editor. Email this page to a colleague
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