Transfer 1 mL Volumes with a Biomek® NX or FX Span-8 System Using Biomek P1000 Tips

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Bob Lund, Beckman Coulter Inc.

Biomek P1000 Tips are now available and supported on Biomek NX and FX Span-8 Systems. These new certified tips offer increased tip capacity for large volume pipetting up to 1 mL, for such applications as:

• DNA (genomic and plasmid) purification from bacterial cell cultures, blood and related sources, body fluids, swabs, and animal and plant tissues, etc.
• Viral titer measurement from blood and related sources
• RNA isolation from bacterial cell cultures, blood and related sources, CSF, and animal and plant tissues, etc.
• Protein purification using HIS-tag chemistry
• Solid phase extraction of target analytes, and for method development, using filtration plates or 1 mL SPE columns
• Broadcast dispensing of reagents for assay development and dilution studies

The P1000 conductive, disposable tips are offered in nonsterile, presterile, and presterile with barrier configurations. The tips are certified to be free of RNase, DNase, DNA (human and mouse)/PCR* inhibition, pyrogen/endotoxin, and trace metals. Compatible labware commonly used with P1000 tips includes: 1 and 2 mL, 96-well deepwell plates; 96-well micro- and amplification plates; 10, 12, & 13 mm diameter test tubes; Microfuge® tubes; 1 and 2 mL SPE filtration plates; and reagent reservoirs.

Use of the P1000 Biomek Tips requires the appropriate Biomek Software and Span-8 Disposal ALP (Automated Labware Positioner) hardware.  Please contact your local Beckman Coulter representative for upgrading Biomek NX and FX Systems.

With Beckman Coulter, you are assured that extensive verification and validation testing has been performed on the P1000 tip to assess both product and system level performance. For the best in system performance, use only Biomek validated tips on Biomek instruments.

Additional Information:

• DS-9897A: Biomek® P1000 Tips--Conductive, Disposable Tips for Biomek NX and FX Span-8 Systems (PDF Format)

For comments or questions about T³ Update, please contact David Daniels, Ph.D., editor.

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