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Automation of Double-Sink PAMPA* Permeability and μSOL Solubility Assays on the Biomek® FX ADMETox Assay Workstation |
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Poster: Yu Suen and Konstantin Tsinman:
Drug discovery in vitro ADMETox assays help reject candidates lacking good pharmaceutical profiles. The automated Double-Sink* PAMPA EVOLUTION96 Assay for permeability and μSOL EVOLUTION96 Assay for solubility are critical ADMETox profiling assays. Methods for these assays have been developed for the Biomek® FX ADMETox Workstation. PAMPA (Parallel Artificial Membrane Permeability Assay), a cost-effective high-throughput method, uses an artificial membrane that models passive transport of epithelial cells. |
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Biomek FX ADMETox Workstation
PAMPA EVOLUTION96
Permeability Assay
μSOL EVOLUTION96 Solubility Assay |
The Double-Sink assay predicts gastrointestinal-tract absorption of candidate drug molecules under different pH conditions. μSOL, a modified shake flask assay, predicts thermodynamic solubility of compounds under different pH conditions. The Biomek ADMETox Workstation with integrated Gut-Box*, filtration and SpectraMax* microplate spectrophotometer provides increased throughput for both assays. The “self-calibration” protocols utilize UV technology enabling high quality data measurement and analysis. These automated PAMPA and μSOL assays on the ADMETox Workstation provide high-throughput, in vitro absorption screening assays for early drug discovery.
Click here for this Poster (PDF)
Additional Information:
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* All trademarks are the property of their respective owners. Where applicable,
the PCR process is covered by patents owned by Roche Molecular Systems, Inc.,
and F. Hoffmann-LaRoche, Ltd.
For Research Use Only; not for use in diagnostic procedures.
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