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Simultaneous Screening for Apoptosis Versus Necrosis by Means of Dual Emission Read-Out Using Multimode Detection |
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Poster: Christoph Wiesner, Rudolf Lucas, Maren Pflueger, Christian Knoll, Wolfgang Schütt, Jim Barry, Josef Atzler nd Harald Hundsberger:
Apoptosis and necrosis are two types of cell death which can be distinguished from each other. Apoptosis is programmed cell death, in which no membrane damage occurs. Necrosis is a form of cell death in which membrane damage leads to the release of cytoplasmic factors causing inflammation. |
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PARADIGM™ Detection Platform |
When screening for anti-tumor factors, it is important to distinguish between apoptotic and necrotic substances, since the latter can cause inflammation and thus major side effects. We can simultaneously measure both types of cell death by using different fluorescently-labelled markers, for apoptotic versus necrotic cell death in transcription-inhibited tumor cells. These markers do not react with viable cells. The detergent Triton X is used as a positive control for necrotic cell death, and Tumor Necrosis Factor (TNF) can be selected as a positive control for apoptosis in a large range of cancer cells. The mechanism of drug-induced cell death can be assessed by using dual wavelength detection.
Click here for this poster (PDF)
Additional Information:
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* All trademarks are the property of their respective owners. Where applicable,
the PCR process is covered by patents owned by Roche Molecular Systems, Inc.,
and F. Hoffmann-LaRoche, Ltd.
For Research Use Only; not for use in diagnostic procedures.
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