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> Nucleic Acid Prep &
Purification > Sequencing
Setup > Sequencing Setup
Biomek®
FX
Sequence Reaction Setup Using the BiomekŪ FX Automated Workstation
Monique Mason, Jason Fawcett, and Laura Pajak, Ph.D.
Beckman Coulter, Inc.
| Method A |  |
| Method B |  |
| Method C |  |
Figure 1. Biomek FX Deck layout for the three sequencing reaction setup methods.
| Location of primers | Aliquot primers? | Location of source DNA | Aliquot source DNA? | Throughput | Method time | |
| Method A | In primer plate | Yes | In source plate | Yes | 6 plates | 6 min 51 sec |
| Method B | In master mix | No | In source plate | Yes | 7 plates | 6 min 25 sec |
| Method C | In master mix | No | In amplification plate | No | 11 plates | 8 min 34 sec |
Table 1. Details of the sequencing reaction setup methods.
Method Flowchart for the sequencing setup methods on the Biomek FX.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
