A Fully Automated High-Throughput Method for the Purification of PCR*
Products on the Biomek® FX Utilizing the QIAGEN QIAquick* Robo96 PCR Clean-up
Kit
Jason Fawcett, Troy Cook, and Paul W. Diaz, Ph.D.
Beckman Coulter, Inc.
Biomek FX Equipment
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 717004 | 1 | Biomek FX Dual Arm System |
| Beckman Coulter, Inc. | 719402 | 1 | Vacuum Valve Unit |
| Beckman Coulter, Inc. | 609674 | 1 | Vacuum Regulator |
| Beckman Coulter, Inc. | 609676 | 1 | Vacuum Tubing Kit |
| Beckman Coulter, Inc. | 717632 | 1 | SPE ALP Platform |
| Beckman Coulter, Inc. | 719229 | 1 | SPE ALP Collar Platform |
| Beckman Coulter, Inc. | 609846 | 1 | Collar Gasket Kit |
| Beckman Coulter, Inc. | 609597 | 1 | Collar, 36 mm |
| Beckman Coulter, Inc. | 717584 | 17 | 1x1 Static ALP Assembly |
| Beckman Coulter, Inc. | 719351 | 1 | Biomek FX NA Prep Wizard Software |
| Beckman Coulter, Inc. | 267689 | 1 | Controller, Software |
| Beckman Coulter, Inc. | 148026 | 2 | Circulating reservoir kit (110V) |
Consumables and Reagents
| Source | Part No. | Quantity | Description |
| QIAGEN, Inc. | 28181 | 1 | QIAquick* 96 PCR clean-up kits |
| Invitrogen | G5018-01 | 1 | E-gels*, 1.2% agarose gels |
| Clontech | K-1912-1 | 1 | Advantaq DNA polymerase |
| Life Technologies | 1561501 | 1 | 1Kb ladder DNA molecular weight markers |
| New England Biolabs | RO137S | 1 | Alu I restriction endonuclease |
| Molecular Probes | P7589 | 11 | PicoGreen* DNA quantification reagents |
| Greiner America, Inc. | 650-101 | 1 | U-bottom 96 well plates |
| Beckman Coulter, Inc. | 372655 | 1 | AP96 200µL tips |
| Beckman Coulter, Inc. | 609801 | 1 | Costar Thermowell 96 plates |
| Beckman Coulter, Inc. | 609844 | 1 | Beckman EIA plates |
Suitable Vacuum Pumps
| Source | Part No. |
Quantity | Description |
| Gast mfg. | 0523U4FG582DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| VWR Scientific | 0323V4AG582DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| VWR Scientific | 0323V4AG21DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Cole Parmer | P-07055-04 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Cole Parmer | P-07055-35 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
Suitable Vacuum Bottles and Cap Pairings
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 975796 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| Beckman Coulter, Inc. | 975797 | 1 | CAP Filling/Venting 1/2" fittings, PP |
| VWR Scientific | 16331-100 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| VWR Scientific | 16225-230 | 1 | Filling/Venting CAP 1/2" fittings, PP |
| Nalgene | 2126-4000 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| Nalgene | 2162-0830 | 1 | Filling/Venting Closure 1/2" fittings, PP |
| Cole Parmer | P-06258-00 | 1 | Filling/Venting CAP 1/2" fittings, PP |
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
