A Fully Automated High-Throughput Method for the Purification of PCR* Products on the Biomek® FX Utilizing the QIAGEN QIAquick* Robo96 PCR Clean-up Kit

Jason Fawcett, Troy Cook, and Paul W. Diaz, Ph.D.
Beckman Coulter, Inc.

Biomek FX system configuration:

• Biomek FX software version 1.5b or higher
• Dual Pod system

Methods:  
Compatible with version 2.0 and higher: QIAquickPCRCleanup2_0.exe

Compatible with version 1.5b: QIAquickPCRCleanup1_5b.exe

To install the QIAquick PCR cleanup method on the Biomek FX:

• Download the self-extracting file containing the methods and import file.
• Double click the file to extract.
• Chose a directory to copy the files.
• Open the Biomek FX software.
• Under the File menu chose Open.
• Select one of the three PCR setup methods.
• If labware definitions are needed follow the additional steps below:

1. In the Biomek FX software under the Tools menu choose Import/Export Utility.
2. Select Open.
3. Select the directory where the import file (.imp) is located and choose Open.
4. Open the Labware folder by clicking the + next to it on the Import File side.
5. Drag and drop the desired setting(s) to the Current Workspace side of the Import/Export Utility. (Note: Dragging an entire folder will copy all of the settings in that folder. You will then have the opportunity of overwriting or not overwriting each individual setting.)
6. Continue to import settings using step 4 until all desired settings are imported.

* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.

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