Automated Methods for PCR* Setup on the Biomek® FX
Lisa Knapp, Jason Fawcett, and Laura Pajak, Ph.D.
Beckman Coulter, Inc.
Biomek FX Equipment
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 717001 | 1 | Biomek FX Single Arm System |
| Beckman Coulter, Inc. | 719368 | 1 | 200µL pipetting head |
| Beckman Coulter, Inc. | 719356 | 1 | Tip loader |
| Beckman Coulter, Inc. | 719357 | 3 | Standard single position ALP |
| Beckman Coulter, Inc. | 719359 | 1 | Standard five position ALP |
| Beckman Coulter, Inc. | 719360 | 1 | High density sixteen position ALP |
| Beckman Coulter, Inc. | 267689 | 1 | Controller with software |
Consumables and Reagents
| Source | Part No. | Quantity | Description |
| Roche Biochemicals | 1277049 | 1 | dNTP reagents for PCR |
| Promega | Q5601 | 1 | M13 forward primer |
| Promega | Q5421 | 1 | M13 reverse primer |
| Greiner America, Inc. | 651161 | 1 | V-bottom plates |
| Greiner America, Inc. | 650101 | 1 | U-bottom plates non-sterile |
| Stratagene | 600280 | 1 | SureStart Taq DNA polymerase |
| Invitrogen | G5018-02 | 1 | 2% E-Gel* agarose gels |
| Roche Biochemicals | 1146165 | 1 | Taq DNA polymerase |
| Beckman Coulter, Inc. | 609801 | 1 | Costar Thermowell 96 plates |
| Beckman Coulter, Inc. | 538619 | 1 | Foil sealers |
| Greiner America, Inc. | 650161 | 1 | U-bottom plate sterile |
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
©1998 - 2006 Beckman Coulter, Inc.
