Increasing Throughput and Quality of Sequencing by Automation of MultiScreen*
Filter Technology on the Biomek® FX
David S. Wexler, Cristina Melero, and Marcy Engelstein
AGY Therapeutics, Inc., South San Francisco, CA 94080
Millipore Corporation, 17 Cherry Hill Drive, Danvers, MA 01923
Millipore Corporation –
www.millipore.com
Biomek FX Equipment
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 717001 | 1 | Biomek FX Single Arm System with POD |
| Beckman Coulter, Inc. | 717632 | 1 | SPE ALP Platform |
| Beckman Coulter, Inc. | 719229 | 1 | SPE Static ALP Collar Platform |
| Beckman Coulter, Inc. | 609674 | 1 | Vacuum Regulator |
| Beckman Coulter, Inc. | 609676 | 1 | Tubing Kit |
| Beckman Coulter, Inc. | 719402 | 1 | Vacuum Valve Kit |
| Beckman Coulter, Inc. | 267689 | 1 | System Software and Controller |
| Beckman Coulter, Inc. | 719367 | 1 | Optional 20µL 96 Pipetting head |
| Beckman Coulter, Inc. | 719368 | 1 | Optional 200µL 96 Pipetting head |
| Beckman Coulter, Inc. | 719715 | 1 | Optional 30µL 384 Pipetting head |
| Beckman Coulter, Inc. | 719356 | 1 | Disposable Tip Loader |
| Beckman Coulter, Inc. | 719363 | 1 | Tip Wash ALP – 96 Channel |
| Beckman Coulter, Inc. | 719366 | 1 | Device Controller |
| Beckman Coulter, Inc. | 719622 | 1 | Optional Tip Wash ALP – 384 Channel |
| Beckman Coulter, Inc. | 719358 | 3 | Standard Three-Position ALP |
| Beckman Coulter, Inc. | 719357 | 5 | Standard Single-Position ALP |
| Beckman Coulter, Inc. | 719347 | 1 | Waste ALP |
Consumables
| Source | Part No. | Quantity | Description |
| Millipore Corporation | SAVM38401 | 1 | Multiscreen 384 vacuum manifold |
| Millipore Corporation | MAVMMEK20 | 1 | MultiScreen manifold assembly kit for Biomek® 2000 and FX workstations |
| Millipore Corporation | S384PCR10 | 1 | MultiScreen384-PCR filter plate |
| Millipore Corporation | S384SEQ10 | 1 | MultiScreen384-SEQ filter plate |
| Beckman Coulter, Inc. | 717251 | 1 | Optional AP96 200µL tips |
| Beckman Coulter, Inc. | 717252 | 1 | Optional AP96 250µL Tip Sterile |
| Beckman Coulter, Inc. | 717253 | 1 | Optional AP96 250µL Barrier Sterile |
| Beckman Coulter, Inc. | 717254 | 1 | Optional AP96 20µL Tips |
| Beckman Coulter, Inc. | 717255 | 1 | Optional AP96 20µL Tips Sterile |
| Beckman Coulter, Inc. | 717256 | 1 | Optional AP96 20µL Barrier Sterile |
| Beckman Coulter, Inc. | 719222 | 1 | Optional AP384 30µL Tips |
| Beckman Coulter, Inc. | 717923 | 1 | Optional AP384 30µL Tips Sterile |
Suitable Vacuum Pumps
| Source | Part No. |
Quantity | Description |
| Millipore Corporation | XX5500000 | 1 | Vacuum/pressure pump 115V/60Hz |
| Gast mfg. | 0523U4FG582DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| VWR Scientific | 0323V4AG582DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| VWR Scientific | 0323V4AG21DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Cole Parmer | P-07055-04 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Cole Parmer | P-07055-35 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
Suitable Vacuum Bottles and Cap Pairings
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 975796 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| Beckman Coulter, Inc. | 975797 | 1 | CAP Filling/Venting 1/2" fittings, PP |
| VWR Scientific | 16331-100 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| VWR Scientific | 16225-230 | 1 | Filling/Venting CAP 1/2" fittings, PP |
| Nalgene | 2126-4000 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| Nalgene | 2162-0830 | 1 | Filling/Venting Closure 1/2" fittings, PP |
| Cole Parmer | P-06258-00 | 1 | Filling/Venting CAP 1/2" fittings, PP |
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
