Increasing Throughput and Quality of Sequencing by Automation of MultiScreen* Filter Technology on the Biomek® FX

David S. Wexler, Cristina Melero, and Marcy Engelstein
AGY Therapeutics, Inc., South San Francisco, CA 94080
Millipore Corporation, 17 Cherry Hill Drive, Danvers, MA 01923
Millipore Corporation – www.millipore.com

Method and Import file: MilliporePCRandSEQcleanup.exe
Note: Make sure you configure the BeckmanPump for light curtain access. Under the Tools menu choose Device Editor. Configure the BeckmanPump with the following settings:

Methods require software version 1.5b or higher.

To install the methods on the Biomek FX:

1. Download the self-extracting file containing the methods and import file.
2. Double click the file to extract.
3. Chose a directory to copy the files.
4. Open the Biomek FX software.
5. Under the File menu chose Open.
6. Select a method to open.
7. If labware definitions are needed follow the additional steps below:
   1. In the Biomek FX software under the Tools menu choose Import/Export Utility.
   2. Select Open.
   3. Select the directory where the import file (.imp) is located and choose Open.
   4. Open the Labware folder by clicking the + next to it on the Import File side.
   5. Drag and drop the desired setting(s) to the Current Workspace side of the Import/Export Utility. (Note: Dragging an entire folder will copy all of the settings in that folder. You will then have the opportunity of overwriting or not overwriting each individual setting.)
   6. Continue to import settings using step d. until all desired settings are imported.

* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.

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