eLabNotebook > Protein Research > SigmaScreen* Biomek® 2000

An Automated Method for Biotinylated Peptide Purification and MALDI Target Spotting for Analysis of Proteins by Mass Spectrometry

Kelly L. Foster, John G. Dapron, Jodi M. Zobrist, Layle K. Barbacci, Tom C. Hassell, and William K. Kappel
Sigma-Aldrich Corp.
Biotechnology Division
P.O. Box 14508, Saint Louis, MO 63178
www.sigmaaldrich.com

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Potential Source of Problem

Possible Solutions

The pipetting onto the MALDI target was not consistent, what can I adjust?

Ensure that your target is flat and that it is sitting level in the (modified) labware holder. You might need to adjust the height of the target by putting a shim of approx. 2 mm to raise the target up enough for the pipette tips to reach the surface. The dispensing height of the MP20 tool will need to be adjusted as well. The rate of dispensing may also be adjusted to offer better pipetting. A slower rate generally ensures more consistent spotting.

* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.

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