An Automated Method for Biotinylated Peptide Purification and MALDI Target Spotting for Analysis of Proteins by Mass Spectrometry
Kelly L. Foster, John G. Dapron, Jodi M. Zobrist, Layle K. Barbacci,
Tom C. Hassell, and William K. Kappel
Sigma-Aldrich Corp.
Biotechnology Division
P.O. Box 14508, Saint Louis, MO 63178
www.sigmaaldrich.com
Biomek 2000 Equipment
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 609000 | 1 | Biomek 2000 |
| Beckman Coulter, Inc. | 267653 | 1 | Controller with BioWorks™ Software |
| Beckman Coulter, Inc. | 609027 | 1 | Eight Channel Wash Tool |
| Beckman Coulter, Inc. | 609056 | 1 | Wash unit with 6 Port Valve |
| Beckman Coulter, Inc. | 609025 | 1 | MP200 Multi Channel Tool |
| Beckman Coulter, Inc. | 609024 | 1 | MP20 Multi Channel Tool |
| Beckman Coulter, Inc. | 609121 | 2 | Tip Rack Holder |
| Beckman Coulter, Inc. | 609120 | 1 | Gray Labware Holder |
| Beckman Coulter, Inc. | 609047 | 1 | Right Side Module |
| Beckman Coulter, Inc. | 609048 | 1 | Left Side Module |
| Beckman Coulter, Inc. | 609751 | 1 | Large Disposal Option |
Consumables
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 609044 | 1 | P20 Tip Nonsterile |
| Beckman Coulter, Inc. | 609042 | 1 | P20 Tip Sterile |
| Beckman Coulter, Inc. | 372654 | 1 | P250 Tip Nonsterile |
| Beckman Coulter, Inc. | 372655 | 1 | P250 Tip Sterile |
| Beckman Coulter, Inc. | 372795 | 1 | Reservoir Frame |
| Beckman Coulter, Inc. | 372790 | 2 | Quarter Reservoir |
| Sigma-Aldrich, Inc. | S6940 | 1 | SigmaScreen™ Streptavidin HC Coated 96 well plate |
| Shimadzu Biotech | DE1487TA OR | 1 | 96 well Axima-CFR MALDI target |
| Shimadzu Biotech | DC7865TA | 1 | Blank Axima-CFR MALDI target |
Suitable Vacuum Pumps
| Source |
Part No. |
Quantity | Description |
| Millipore Corporation | XX5500000 | 1 | Vacuum/Pressure pump 115V/60Hz |
| Gast mfg. | 0523U4FG582DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| VWR Scientific | 0323V4AG582DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| VWR Scientific | 0323V4AG21DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Cole Parmer | P-07055-04 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Cole Parmer | P-07055-35 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
Suitable Vacuum Bottles and Cap Pairings
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 975796 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| Beckman Coulter, Inc. | 975797 | 1 | CAP Filling/Venting 1/2" fittings, PP |
| VWR Scientific | 16331-100 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| VWR Scientific | 16225-230 | 1 | Filling/Venting CAP 1/2" fittings, PP |
| Nalgene | 2126-4000 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| Nalgene | 2162-0830 | 1 | Filling/Venting Closure 1/2" fittings, PP |
| Cole Parmer | P-06258-00 | 1 | Filling/Venting CAP 1/2" fittings, PP |
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
