An Automated Method for Biotinylated Peptide Purification and MALDI Target Spotting for Analysis of Proteins by Mass Spectrometry
Kelly L. Foster, John G. Dapron, Jodi M. Zobrist, Layle K. Barbacci,
Tom C. Hassell, and William K. Kappel
Sigma-Aldrich Corp.
Biotechnology Division
P.O. Box 14508, Saint Louis, MO 63178
www.sigmaaldrich.com
Figure 1. Biomek 2000 Deck Layout for Peptide Purification using the SigmaScreen™ Streptavidin
HC Coated 96 well plate
Method Flowchart for the Peptide Purification Assay using the SigmaScreen
Streptavidin HC Coated 96 well plate
(click the image to enlarge).
Figure 2. Labware holder modification diagram for the Peptide Purification
protocol on the Biomek 2000
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
