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Sigma HIS-Select* iLAP* Biomek® FX
Sigma's HIS-Select* iLAP* Plates for Integrated Lysis and Purification
of Recombinant Histidine-tagged Proteins from Bacteria on the Biomek®
FX
Sigma-Aldrich Corp.
Biotechnology Division
P.O. Box 14508, Saint Louis, MO 63178
www.sigmaaldrich.com
Configuring the Deck for HIS-Select iLAP Plates
Instrument configurations, deck definitions, or numbering of deck positions
may vary from those used at Sigma-Aldrich in development of these methods.
To run these methods, some labware rearrangement may be required.
Figure 1. Biomek FX Deck Layout for iLAP_BCA_Quantification Method
| Deck Position | Equipment |
| P1 | 96-well deep well plate containing cells in medium |
| P2 | 96-well reservoir for BCA Working Solution |
| P3 | AP96 P250 tip box for water (fresh) |
| P4 | 12-column reservoir for waste |
| P5 | AP96 P250 tip box for BCA Working Solution |
| P6 | AP96 P250 tip box for water (waste) |
| P7 | BCA standard curve plate with plate |
| P8 | HIS-Select iLAP plate with plate lid |
| P9 | 12-column reservoir for water |
| P10 | AP96 P250 tip box for TBST (waste) |
| P11 | AP96 P250 tip box for TBST (fresh) |
| P12 | AP96 P250 tip box for cells in medium |
| P13 | 12-column reservoir for TBST |
| P15 | Span-8 200 µl tip box for BSA dilution |
Figure 2. Biomek FX Deck Layout for iLAP_Elution_Laemmli Method
| Deck Position | Equipment |
| P1 | 96-well deep well plate containing cells in medium |
| P2 | 96-well reservoir for 2x Laemmli buffer |
| P3 | AP96 P250 tip box for TBST waste |
| P4 | 12-column reservoir for waste |
| P5 | AP96 P250 tip box for 2x Laemmli buffer |
| P6 | 96-well reservoir for elution solution |
| P7 | AP96 P250 tip box for water |
| P8 | HIS-Select iLAP plate with plate lid |
| P9 | 12-column reservoir for water |
| P10 | AP96 P250 tip box for water (fresh) |
| P11 | AP96 P250 tip box for TBST (fresh) |
| P12 | AP96 P250 tip box for cells in medium |
| P13 | 12-column reservoir for TBST |
| P15 | Span-8 200 µl tip box for cells in medium |
* All trademarks are the property of their respective owners. Where applicable,
the PCR process is covered by patents owned by Roche Molecular Systems,
Inc., and F. Hoffman-LaRoche, Ltd.
