eLabNotebook - Promega MagneSil* ONE Biomek® FX

eLabNotebook > Protein Research > Promega MagneHis* Biomek® FX

Promega's Automated MagneHis* Protein Purification System on the Biomek® FX

Promega Corporation – www.promega.com

Product Components

Product Size Cat.#

MagneHis* Protein Purification System 10ml V8550

Includes:

• 250ml MagneHis* Binding/Wash Buffer

• 50ml MagneHis* Elution Buffer

• 60ml FastBreak* Cell Lysis Reagent, 10X

• 10ml MagneHis* Ni-Particles

• 2 vials DNase I (lyophilized)

• 1 Protocol

Storage Conditions: Store all MagneHis* Protein Purification System components at 4°C. Do not freeze the MagneHis* Ni-Particles. The DNase I (lyophilized) may be stored at room temperature. Upon resuspension in water, store the DNase I in aliquots at –20°C. For convenience, the resupended DNase I may be stored at 4°C for up to one week. FastBreak* Cell Lysis Reagent may form a precipitate at low temperature. If this occurs, warm the reagent to room temperature before use.

Automated Processing Requirements for the Beckman Biomek® FX

A. Instrument Requirements for the Biomek® FX

Source	Part No.	Quantity	Description
Beckman Coulter, Inc.	717001	1	Biomek® FX Workatation
Beckman Coulter, Inc.	267689	1	Minimum: Biomek® FX software version 2.5
Beckman Coulter, Inc.	719368	1	96-channel POD
Beckman Coulter, Inc.	719357	11	Labware positions for 1 POD
Beckman Coulter, Inc.	719356	1	Tip loader ALP
Beckman Coulter, Inc.	379410	1	Orbital shaker ALP (optional)

B. Labware Requirements for the Biomek® FX

Source	Part No.	Quantity	Description
Promega Corporation	V3031	1	Deep Well MagnaBot* 96 Magnetic Separation Device
Marsh	AB-0932	2	2.2ml deep-well plate or comparable
Promega Corporation	Z3301	1	1/4 inch Foam Spacer
Marsh	AB-0564	1	1.2ml deep-well plate (or comparable)
Innovative Microplate	S30014	3	Pyramid-bottom 96-well reservoirs (or comparable)
Promega Corporation	A9161	2	Polystyrene U-bottom multiwell plates (or comparable)
Beckman Coulter, Inc.	717253	3	200 tips-barrier (rack)

* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.