Biomek® 2000
Beckman Coulter Application (system and method support)
A Fully Automated Method for Purification of PCR* Products on the Biomek® 2000 Laboratory Workstation Utilizing the QIAGEN QIAquick* 96 PCR Clean-up Kit
Troy Cook, Jason Fawcett, Graham Threadgill, Ph.D., and Paul W. Diaz,
Ph.D.
Automated Solutions Development Center
Beckman Coulter, Inc.
Many of the processes occurring downstream of PCR* require the separation of free or spent reaction components such as nucleotides, primers and buffers from the PCR product; the sheer volume of which can quickly overwhelm any laboratory utilizing manual methods. Thus, there is a strong need for high throughput methods of PCR product purification. To address these needs we present here a fully automated BioWorks™ method of PCR product purification on the Biomek 2000 utilizing the QIAGEN QIAquick 96 PCR Clean-up kit.
Features
- Purified up to 96 PCR products in 30 minutes.
- No need to modify method if using reaction volumes between 10 to 100µL.
- Utilizes pattern to easily choose any number of samples up to 96.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
