Sequencing Cleanup Biomek® 2000
Customer Application (system support)
High-Throughput Purification of BigDye* Terminator Fluorescent DNA Sequencing Reactions using the Wizard* MagneSil* System on Beckman Coulter's Biomek® 2000 Automated Laboratory Workstation
Paul Otto**, Michael Bjerke**, Steve Krueger**, Steve Ekenberg**, Keith
Roby**, Matthew Cu**, Chad Pittman**, Laura Pajak, Ph.D.**
Beckman Coulter, Inc.
**Indianapolis, IN and ***Fullerton, CA
Promega Corporation – www.promega.com
Poster Abstract
Automated fluorescent sequencing using ABI PRISM* BigDye* Terminator
Chemistry is a common method for high-throughput DNA sequence determination.
Common purification methods are based on gel filtration and/or precipitation
by ethanol. Promega has developed a series of ‘silica-paramagnetic’
particles (MagneSil) for the purification of nucleic acids from reaction
components in the BigDye Terminator DNA Sequencing reactions prior to
automated sequence analysis. This removal prior to analysis enables clean
data collection. Sequence quality is similar to or better than the manual
methods and offers the distinct advantage of a truly hands-off protocol.
Details of Beckman Coulter’s Biomek 2000 Laboratory Automation Workstation
methods and data generated from these methods are discussed in this poster.
* All trademarks are the property of their respective owners. Where applicable,
the PCR process is covered by patents owned by Roche Molecular Systems,
Inc., and F. Hoffman-LaRoche, Ltd.
©1998 - 2006 Beckman Coulter, Inc.
