Fully-Automated Method for Purifying PCR* Products using
Promega's Wizard MagneSil* PCR Clean-Up System on the Biomek® FX
Liquid Handling System
**Chad Pittman, ***Graham Threadgill, Ph.D., and **Laura Pajak, Ph.D.
Beckman Coulter, Inc.
**Indianapolis, Indiana and ***Fullerton, California
Brad Larson and Paul Otto
Promega Corporation - www.promega.com
Madison, Wisconsin
Biomek FX Equipment
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 717013 | 1 | Biomek FX Dual Arm System |
| Beckman Coulter, Inc. | 379448 | 1 | Biomek FX Orbital Shaking ALP |
| Beckman Coulter, Inc. | 379447 | 1 | Biomek FX Magnetic ALP |
| Beckman Coulter, Inc. | 379511 | 1 | 96-Well Post Magnet for Magnetic ALP |
| Beckman Coulter, Inc. | 719357 | 2 | Standard Single-Position ALP |
| Beckman Coulter, Inc. | 719347 | 1 | Waste ALP |
| Beckman Coulter, Inc. | 719948 | 1 | High-Density Twelve-Position 4x3 ALP |
| Beckman Coulter, Inc. | 719363 | 1 | 96-Channel Tip Wash ALP(110V) |
| Beckman Coulter, Inc. | 719662 | 1 | 96-Channel Tip Wash ALP(220V) |
| Beckman Coulter, Inc. | 719368 | 1 | 200µL 96-Channel Disposable Tip Pipetting Head |
| Beckman Coulter, Inc. | 719356 | 1 | Tip Loader ALP |
| Beckman Coulter, Inc. | 719366 | 1 | Device Controller |
| Beckman Coulter, Inc. | 719349 | 1 | Biomek FX System Software |
| Beckman Coulter, Inc. | 609883 | 1 | Automation PC controller |
| Beckman Coulter, Inc. | 977493 | 1 | CRT Flatpanel color 15" monitor |
| Beckman Coulter, Inc. | 719807 | 1 | Span-8 Fixed Tip Teflon Coated Qty. 8 |
Consumables
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 717251 | 1 | AP96 P250 Tips (Case of 10 Racks) |
| Promega, Inc. | V8151 | 1 | MagnaBot* 96 Magnetic Separation Device |
| Promega, Inc. | V8381 | 1 | MagnaBot* Spacer |
| Innovative Microplate, Inc. | S30014 | 1 | 96-Channel Reservoir with Pyramid bottom 25/case |
| Innovative Microplate, Inc. | S30019 | 1 | Twelve Column Reservoir 25/case |
| Greiner Bio-One, Inc. | 650101 | 2 | 96-Well U-Bottom Plates 5/bag |
| Greiner Bio-One, Inc. | 652280 | 1 | 96-Well Half Skirted PCR plate, 10/BAG |
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
