Beckman Coulter Application (system and method support)
Fully-Automated Method for Purifying PCR* Products using the Wizard* MagneSil* PCR Cleanup System on the Biomek® 2000 Laboratory Automation Workstation
Chad Pittman**, Graham Threadgill***, Ph.D., and Laura Pajak**, Ph.D.
Beckman Coulter, Inc.
**Indianapolis, IN and ***Fullerton, CA
Brad Larson, Paula Brisco, Steve Krueger, Steve Ekenberg, Elaine Schenborn,
Ph.D., and Paul Otto
Promega Corporation – www.promega.com
The abundant use of PCR products in high-throughput sequencing and microarray research provides the need for automated purification methods. Previous methodologies have employed either centrifugation or vacuum filtration with filter plates to capture, purify and eventually elute PCR products away from excess primers, unincorporated dNTPs and salt contaminants.
MagneSil paramagnetic particles have been adapted for purification of amplification products thereby eliminating the need for vacuum collars and filter plates as the process can be performed in standard microplates.
The magnetic particles can be manipulated (aspirated and dispensed) on robotic platforms making them particularly suitable for automation. Furthermore, the small particle size ensures rapid sedimentation when placed in a magnetic field. Methods for automated purification of PCR products on the Biomek 2000 were created to aid scientists in performing these applications.
Features
- Utilizes an integrated DPC MicroMix* 5 orbital shaker to efficiently resuspend the paramagnetic particles.
- Single-stranded primers and double-stranded DNA of 150bp or less will not bind to MagneSil particles.
- Excellent recovery of sample from the beads – greater than 92% recovery.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
©1998 - 2006 Beckman Coulter, Inc.
