eLabNotebook - Sigma’s Extract-N-Amp* Biomek® FX

Sigma’s Extract-N-Amp* Tissue PCR Kits for Isolating DNA from Mouse Tail Clippings and Other Animal Tissue Samples on the Biomek® FX

Sigma-Aldrich Corp.
Biotechnology Division
P.O. Box 14508, Saint Louis, MO 63178
www.sigmaaldrich.com

Instrument configurations, deck definitions, or numbering of deck positions may vary from those used at Sigma-Aldrich in development of these methods. To run these methods, some labware rearrangement may be required. The deck layout is provided below as a guide.

Figure 1. Deck Layout for on the Biomek FX for the Extract-N-Amp Tissue Application

Deck Position Equipment

TL1 AP96 P250 Barrier Tips, Sterile

P2 AP96 P250 Barrier Tips, Sterile

P3 Swap

P4 96 well reservoir for the mixture of Extraction and Tissue Preparation Solution

P5 AP96 P250 tip box for BCA Working Solution

P6 Lid

P7 96 well PCR plate with full skirt containing tissue samples

P8 96 well reservoir for Neutralization Solution

P11 96 well PCR plate with full skirt for transferring neutralized tissue extracts

P12 96 well PCR plate with half skirt for PCR reaction setup (seated into a
plate holder)

P13 12 column reservoir for PCR Master Mix

P14 Span-8 P250 Barrier Tips

P15 Span-8 P20 Barrier Tips

P16 24 position Eppendorf® IsoTherm system (DNA Control)