A Wizard Approach to Generating High Quality Plasmid DNA on the Biomek®
FX Using the WIZARD* SV96 Plasmid DNA Purification System from Beckman
Coulter
Monique Mason, John Snider, Graham J. Threadgill, Ph.D., and Laura Pajak,
Ph.D.
Beckman Coulter, Inc.
Biomek FX Equipment
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 717001 | 1 | Biomek FX Single Arm System with POD |
| Beckman Coulter, Inc. | 719368 | 1 | 96 channel head 200µL |
| Beckman Coulter, Inc. | 719349 | 1 | System Software |
| Beckman Coulter, Inc. | 719824 | 1 | Two Plate Miniprep Wizard |
| Beckman Coulter, Inc. | 719356 | 1 | Tip Loader |
| Beckman Coulter, Inc. | 719357 | 10 | 1x1 ALP |
| Beckman Coulter, Inc. | 719359 | 1 | 1x5 ALP |
| Beckman Coulter, Inc. | 719347 | 1 | Trash ALP |
| Beckman Coulter, Inc. | 719804 | 1 | 1x1 (with indented sides) ALP |
| Beckman Coulter, Inc. | 719750 | 1 | Filtration system |
| Beckman Coulter, Inc. | 719363 | 1 | Tip Wash |
| Beckman Coulter, Inc. | 609883 | 1 | Automation Controller |
| Beckman Coulter, Inc. | 974571 | 1 | 17” monitor |
Consumables
| Source | Part No. | Quantity | Description |
| Costar | 3596 | 1 | 96 Well Cell Culture Cluster Lid |
| Beckman Coulter, Inc. | 608133 | 1x96 preps | Wizard SV 96 Plasmid DNA Purification System |
| Beckman Coulter, Inc. | 608134 | 5x96 preps | Wizard SV 96 Plasmid DNA Purification System |
| Beckman Coulter, Inc. | 608136 | 10 pack | Wizard SV 96 DNA Binding Plates |
| Beckman Coulter, Inc. | 608137 | 10 pack | Wizard SV 96 Lysate Clearing Plates |
| Beckman Coulter, Inc. | 608138 | 500ml | Wizard SV 96 Cell Resuspension Solution |
| Beckman Coulter, Inc. | 608139 | 500ml | Wizard SV 96 Cell Lysis Solution |
| Beckman Coulter, Inc. | 608140 | 500ml | Wizard SV 96 Neutralization Solution |
| Beckman Coulter, Inc. | 608141 | 185ml | Wizard SV 96 Wash Solution |
| Beckman Coulter, Inc. | 717251 | 1 case | AP96 200µL tips |
Suitable Vacuum Pumps
| Source | Part No. |
Quantity | Description |
| Gast mfg. | 0523U4FG582DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| VWR Scientific | 0323V4AG582DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| VWR Scientific | 0323V4AG21DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Cole Parmer | P-07055-04 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Cole Parmer | P-07055-35 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
Suitable Vacuum Bottles and Cap Pairings
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 975796 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| Beckman Coulter, Inc. | 975797 | 1 | CAP Filling/Venting 1/2" fittings, PP |
| VWR Scientific | 16331-100 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| VWR Scientific | 16225-230 | 1 | Filling/Venting CAP 1/2" fittings, PP |
| Nalgene | 2126-4000 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| Nalgene | 2162-0830 | 1 | Filling/Venting Closure 1/2" fittings, PP |
| Cole Parmer | P-06258-00 | 1 | Filling/Venting CAP 1/2" fittings, PP |
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
