High Throughput Generation of Quality Plasmid DNA on the Biomek® FX using
the Wizard* SV 96 Plasmid DNA Purification System from Beckman Coulter,
Inc.
Jason Fawcett, Monique Mason, and Laura Pajak, Ph.D.
Beckman Coulter, Inc.
Biomek FX Equipment
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 717004 | 1 | Biomek FX Dual Arm System |
| Beckman Coulter, Inc. | 719769 | 1 | Biomek FX Left Filtration System |
| Beckman Coulter, Inc. | 719768 | 1 | Biomek FX Right filtration System |
| Beckman Coulter, Inc. | 719357 | 6 | Standard Single-Position ALP |
| Beckman Coulter, Inc. | 719877 | 1 | Biomek FX NA Prep Wizard Software |
| Beckman Coulter, Inc. | 267689 | 1 | Controller & Software |
| Beckman Coulter, Inc. | 719368 | 2 | 200µL Pipetting Head |
| Beckman Coulter, Inc. | 148026 | 2 | Circulating reservoir kit (110V) |
| Beckman Coulter, Inc. | 148520 | 1 | Left Stacker Carousel |
| Beckman Coulter, Inc. | 148521 | 1 | Right Stacker Carousel |
| Beckman Coulter, Inc. | 148522 | 8 | 10 Position Stack |
| Beckman Coulter, Inc. | 719363 | 2 | Tip Wash ALP 96-Channel |
| Beckman Coulter, Inc. | 719366 | 1 | Device Controller |
| Beckman Coulter, Inc. | 719347 | 2 | Waste ALP |
Consumables
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 717251 | 3 | AP96 P250 Tips (Case of 10 Racks) |
| Invitrogen | G5018-1 | 5 | 1.2% Agarose E-Gels |
| Beckman Coulter, Inc. | 373660 | 1 | 96 Well Flat Bottom Plate |
| Costar | 3596 | 12 | 96 Well Cell Culture Cluster Lid |
| Beckman Coulter, Inc. | 608134 | 3 | Wizard SV96 Plasmid Kit |
| Promega Corporation | R6011 | 1 | Eco R1 |
| Promega Corporation | R6071 | 1 | Bgl I |
| Promega Corporation | R6331 | 1 | Pvu II |
Suitable Vacuum Pumps
| Source | Part No. |
Quantity | Description |
| Gast mfg. | 0523U4FG582DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| VWR Scientific | 0323V4AG582DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| VWR Scientific | 0323V4AG21DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Cole Parmer | P-07055-04 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Cole Parmer | P-07055-35 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
Suitable Vacuum Bottles and Cap Pairings
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 975796 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| Beckman Coulter, Inc. | 975797 | 1 | CAP Filling/Venting 1/2" fittings, PP |
| VWR Scientific | 16331-100 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| VWR Scientific | 16225-230 | 1 | Filling/Venting CAP 1/2" fittings, PP |
| Nalgene | 2126-4000 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| Nalgene | 2162-0830 | 1 | Filling/Venting Closure 1/2" fittings, PP |
| Cole Parmer | P-06258-00 | 1 | Filling/Venting CAP 1/2" fittings, PP |
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
