eLabNotebook - Sigma's Automated Protocol for Extract-N-Amp* Plant PCR* Kits on the Biomek® FX

eLabNotebook > Nucleic Acid Prep & Purification > PCR Cleanup > Sigma Extract-N-Amp* Plant PCR
Biomek® FX

Sigma's Automated Protocol for Extract-N-Amp* Plant PCR* Kits on the Biomek® FX

Sigma-Aldrich Corporation – www.sigmaaldrich.com

Product Components

Reagents
Provided Product Code Extract-N-Amp
Plant
XNAR REDExtract-N-Amp
Plant
XNAPR SYBR Green
Extract-N-Amp
Plant
XNAPRG

Package Size 1,000 extractions
1,000 amplifications 1,000 extractions
1,000 amplifications 1,000 extractions
1,000 amplifications

Extraction
Solution E7526 120 ml 120 ml 120 ml

Dilution
Solution D5688 120 ml 120 ml 120 ml

Extract-N-Amp PCR Ready Mix or SYBR Green Extract-N-Amp PCR Ready Mix E3004 (for XNAR) R4775 (for XNAPR) S4320 (for XNAPRG) 12 ml 12 ml 12 ml

The Extract-N-Amp Plant PCR Kits can be stored at 2-8 °C for up to 3 weeks. For long-term storage, store at –20 °C. Do not store in a frost-free freezer.

Materials to be Supplied by the User

Source

Part No.

Quantity

Description

Sigma-Aldrich G2N10, G2N70, and G2N250
1
(Optional) GenElute* Plant Genomic DNA Miniprep Kit for use as genomic DNA control

Sigma-Aldrich W4502
1
Water, molecular biology reagent

Sigma-Aldrich P4616
1
96-well PCR plates, with full skirt

ABgene AB-1100
1
96-well PCR plates, with half skirt

Fisher 07200694
1
Lid, universal

ABgene AB-0866
1
Ultra clear cap strip

Corning 6525
1
Corning plate holder

Sigma-Aldrich Z369659
1
Sealing film, SealPlate

Fisher 05-405-22
1
24-position Eppendorf* IsoTherm System

Innovative Microplates S30028
1
12-column reagent reservoir with low profile

Innovative Microplates S30018
1
96-well reservoir with low profile and pyramidal bottom

Innovative Microplates S30019
1
(Optional) 12-column reagent reservoir with high profile

Innovative Microplates S30014
1
(Optional) 96-well reservoir with high profile and pyramidal bottom

Fisher 15-077-26
1
Thermometer

Customer Supplied N/A
1
Plant leaf tissues

Customer Supplied N/A
1
Paper punch (standard one-hole)

Customer Supplied N/A
1
Forceps (small to medium in size)

Customer Supplied N/A
1
Microcentrifuge tubes (1.5 ml, 2 ml screw cap)

Customer Supplied N/A
1
Thermal Cycler

Instrument Requirements for the Biomek FX Workstation

Source

Part No.

Quantity

Description

Beckman Coulter, Inc. Beckman Coulter
1
Orbital Shaker

Beckman Coulter, Inc. Beckman Coulter
1
Peltier ALP

Beckman Coulter, Inc. Beckman Coulter
1
Multichannel Pod (96 Mandrel 200 µl Head)

Beckman Coulter, Inc. Beckman Coulter
1
Span-8 Pod (1 ml Syringe)

Beckman Coulter, Inc. Beckman Coulter
1
Gripper

Beckman Coulter, Inc. Beckman Coulter
1
Tip Loader

Beckman Coulter, Inc. Beckman Coulter
1
Span-8 Tip Trash

Beckman Coulter, Inc. Beckman Coulter
1
Span-8 Tip Trash

Beckman Coulter, Inc. Beckman Coulter
4
Standard Passive ALPs (One by Three)

Beckman Coulter, Inc. Beckman Coulter
3
Standard Passive ALPs (One by One)

Beckman Coulter, Inc. 717253
2
AP96 P250 Barrier Tips, Sterile

Beckman Coulter, Inc. 717256
1
AP96 P20 Barrier Tips, Sterile

Beckman Coulter, Inc. 379503
1
Span-8 P250 Barrier Tips, Sterile

Beckman Coulter, Inc. 379506
1
Span-8 P20 Barrier Tips, Sterile

* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.