Biomek® 2000
A Fully Automated Method for Purification of Plasmid DNA on the Biomek® 2000
Laboratory Workstation Utilizing the QIAGEN QIAprep* 96 Turbo Miniprep
Kit
Troy Cook, Ryan McCrae, and Paul W. Diaz, Ph.D.
Beckman Coulter, Inc.
Biomek 2000 Equipment
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 609000 | 1 | Biomek 2000 Instrument |
| Beckman Coulter, Inc. | 267653 | 1 | Controller with BioWorks™ software |
| Beckman Coulter, Inc. | 609047 | 1 | Right side module |
| Beckman Coulter, Inc. | 609048 | 1 | Left side module |
| Beckman Coulter, Inc. | 609001 | 1 | Gripper Tool System w/disposal option |
| Beckman Coulter, Inc. | 267615 | 1 | 96-filtration system |
| Beckman Coulter, Inc. | 609027 | 1 | Eight-Channel Wash tool |
| Beckman Coulter, Inc. | 609056 | 1 | Wash Unit with 6 port valve |
| Beckman Coulter, Inc. | 609025 | 1 | MP200 Multi Channel Tool |
| Beckman Coulter, Inc. | 609803 | 1 | 65mm Collar with spacer plate and reservoir holder |
| Beckman Coulter, Inc. | 609736 | 2 | Labware collar holders |
| Beckman Coulter, Inc. | 609846 | 1 | Neoprene Gasket Kit, 5 pcs. |
| Beckman Coulter, Inc. | 609676 | 1 | Tubing kit 10', filtration system |
| Beckman Coulter, Inc. | 609687 | 1 | Tubing kit, wash unit |
| Beckman Coulter, Inc. | 879049 | 1 | Vacuum grease 1 oz. |
| Beckman Coulter, Inc. | 609120 | 4 | Gray Labware Holder |
| Beckman Coulter, Inc. | 609121 | 2 | Tip Rack Holders |
Consumables and Reagents
| Source | Part No. | Quantity | Description |
| QIAGEN, Inc. | 27191 | 1 | QIAprep* plasmid purification kits |
| Invitrogen | G5018-01 | 1 | E-gels*, 1.2% agarose gels |
| Clontech | K-1912-Y | 1 | Advantaq DNA polymerase |
| Life Technologies | 15615016 | 1 | 1Kb ladder DNA molecular weight markers |
| New England Biolabs | R0101S | 1 | EcoR I restriction endonuclease |
| New England Biolabs | R0143S | 1 | Bgl I restriction endonuclease |
| New England Biolabs | R0151S | 1 | Pvu II restriction endonuclease |
| Beckman Coulter, Inc. | 372655 | 1 | AP96 200µL tips |
| Beckman Coulter, Inc. | 372654 | 1 | Biomek 2000 P250 tips |
| Beckman Coulter, Inc. | 538619 | 1 | SEAL & SAMPLE* foil lids |
Suitable Vacuum Pumps
| Source | Part No. |
Quantity | Description |
| Gast mfg. | 0523U4FG582DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| VWR Scientific | 0323V4AG582DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| VWR Scientific | 0323V4AG21DX | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Cole Parmer | P-07055-04 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
| Cole Parmer | P-07055-35 | 1 | Rotary Oil Vacuum Pump 115VAC 60Hz |
Suitable Vacuum Bottles and Cap Pairings
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 975796 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| Beckman Coulter, Inc. | 975797 | 1 | CAP Filling/Venting 1/2" fittings, PP |
| VWR Scientific | 16331-100 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| VWR Scientific | 16225-230 | 1 | Filling/Venting CAP 1/2" fittings, PP |
| Nalgene | 2126-4000 | 1 | Heavy-duty Bottle, HDPP 4 Liter |
| Nalgene | 2162-0830 | 1 | Filling/Venting Closure 1/2" fittings, PP |
| Cole Parmer | P-06258-00 | 1 | Filling/Venting CAP 1/2" fittings, PP |
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
