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Purification > Promega MagneSil*
Total
RNA mini-Isolation Biomek® FX
Promega's MagneSil* Total RNA mini-Isolation System on the Biomek® FX
Promega Corporation – www.promega.com
Product Components
| Product | Size | Cat.# |
| MagneSil* Total RNA mini-Isolation System | 4 plate | Z3351 |
total RNA from up to four 96- or 384-well plates. Includes:
• 100ml RNA Lysis Buffer (RLA), 100ml
• 20ml MagneSil* RNA Paramagnetic Particles (PMPs)
• 4 vials DNase I (lyophilized)
• 25ml Yellow Core Buffer
• 5ml MnCl2, 0.09M
• 26.5ml DNase Stop Solution (concentrated)
• 150ml Nuclease-Free Water
• 1 Protocol
Storage Conditions: Store the MagneSil* Total RNA mini-Isolation System at room temperature
(22 to 25°C). Do not refrigerate or freeze the MagneSil* RNA PMPs.
Available Separately
The products listed below are required for isolation of RNA using the MagneSil* Total RNA mini-Isolation System but must be purchased separately.
96-well Purification
| Product | Cat.# |
| MagnaBot* 96 Magnetic Separation Device | V8151 |
| 1/4 inch Foam Spacer | Z3301 |
| Collection Plates (4 × 96-well U-bottom plates) | A9161 |
384-well Purification
| Product | Cat.# |
| MagnaBot* 384 Magnetic Separation Device* | V8241 |
| 384-well Plate, Flat | V5291 |
Instrument Requirements
The following is a list of Beckman Coulter parts that are required to use the MagneSil* Total RNA mini-Isolation System on the Biomek® FX Workstation.
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 267689 | Minimum: Biomek® FX Software Version 2.1 | |
| Beckman Coulter, Inc. | 719368 | 96-channel POD | |
| Beckman Coulter, Inc. | 380560 | Orbital Shaker | |
| * Minimum number of labware positions accessible by 1 POD | |||
| Beckman Coulter, Inc. | 719368 | For 96-well purification | |
| Beckman Coulter, Inc. | 719368 | For 384-well purification | |
Materials to be Supplied by the User for 96-well Purification
| Source | Part No. | Quantity | Description |
| Innovative Microplate | S30014 | 1 |
Pyramid-bottom reservoir plates |
| Promega Corporation | A9161 | 1 |
96-well U-bottom Collection Plates |
| Axygen Scientific | FXF-180-LRS | 1 |
Biomek® FX P250 barrier tips |
| Marsh Bio Products | AB0932 | 1 |
Deep Well Plate, 2.2ml |
| Greiner | 650201 | 1 |
96-well U-bottom polypropylene plate (for eluted purifed total RNA) |
| Promega Corporation | V8151 | 1 |
MagnaBot® 96 Separation Device |
| Promega Corporation | Z3301 | 1 |
1/4 inch Foam Spacer |
Materials to be Supplied by the User for 384-well Purification
| Source | Part No. | Quantity | Description |
| Innovative Microplate | S30014 | Pyramid-bottom reservoir plates | |
| Promega Corporation | A9161 | 96-well U-bottom Collection Plates | |
| Axygen Scientific | FXF-180-LRS | Biomek® FX P250 barrier tips | |
| Marsh Bio Products | AB0932 | 1 | Deep Well Plate, 2.2ml |
| Promega Corporation | V8241 | 1 | MagnaBot® 384Separation Device |
| Promega Corporation | V5291 | 1 | 384-well Plate, Flat (for eluted purified total RNA) |
* All trademarks are the property of their respective owners. Where applicable,
the PCR process is covered by patents owned by Roche Molecular Systems,
Inc., and F. Hoffman-LaRoche, Ltd.
