eLabNotebook
> Nucleic Acid Prep &
Purification > Plasmid
DNA Purification > Promega MagneSil
Tfx*
Biomek® FX
Promega's Wizard MagneSil Tfx* System on the Biomek® FX
Promega Corporation – www.promega.com
Product Components
| Product | Size | Cat.# |
| Wizard MagneSil Tfx* System | 4 × 96 preps | A2380 |
• 150ml Cell Resuspension Solution
• 150ml Cell Lysis Solution
• 150ml Neutralization Solution
• 38ml MagneSil* BLUE
• 19ml Endotoxin Removal Resin
• 30ml MagneSil* RED
• 38ml 4/40 Wash Solution
• 2 × 25ml Nuclease-Free Water
• 1 Protocol
| Product | Size | Cat.# |
| Wizard MagneSil Tfx* System | 8 × 96 preps | A2381 |
Each system contains sufficient reagents for 8 × 96-well plates. Includes:
• 2 × 150ml Cell Resuspension Solution
• 2 × 150ml Cell Lysis Solution
• 2 × 150ml Neutralization Solution
• 2 × 38ml MagneSil* BLUE
• 2 × 19ml Endotoxin Removal Resin
• 2 × 30ml MagneSil* RED
• 2 × 38ml 4/40 Wash Solution
• 4 × 25ml Nuclease-Free Water
• 1 Protocol
Storage Conditions: Store all Wizard MagneSil Tfx* System
components at 22–25°C. See the product label for expiration date.
Materials to Be Supplied by the User:
(Solution compositions are provided in Section VII.A.)
In addition to the ALPs needed for the Biomek® FX robotic workstation,
the following items are needed to perform plasmid DNA isolation with the
workstation:
| Source | Part No. | Quantity | Description |
| User Supplied | N/A | 1 |
Culture medium containing appropriate antibiotic |
| Beckman Coulter, Inc. | 362701 | 1 |
Avanti® J2HC Centrifuge |
| Promega Corporation | V8151 | 1 |
MagnaBot* 96 Magnetic Separation Device |
| Promega Corporation | Z3271 | 1 |
Heat Transfer Block |
| Innovative Microplate | S30014 | 1 |
4 x 96-well reservoirs, pyramid bottom |
| Note: The Innovative Microplate reservoirs are ideal, as their structure allows a low dead volume. If a different reservoir is used, larger dead volumes may necessitate re-using solutions. |
This protocol requires a Biomek® FX robotic workstation with the following ALPs:
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 719856 | 1 |
Tip Loader ALP |
| Beckman Coulter, Inc. | 719804 | 1 |
96-well Tipwash ALP |
| Beckman Coulter, Inc. | 719360 | 1 |
3 × single-position labware ALP |
| Beckman Coulter, Inc. | 719360 | 1 |
16-position labware ALP |
| User Supplied | N/A | 1 |
circulating water bath that heats to 55–65°C |
| Beckman Coulter, Inc. | 719368 | 1 |
Single Robotic Arm fitted with a 96-well Pipetting Head |
| Beckman Coulter, Inc. | 719361 | 1 |
heating/cooling ALP |
| Beckman Coulter, Inc. | 719352 | 1 |
linear shaker ALP |
* All trademarks are the property of their respective owners. Where applicable,
the PCR process is covered by patents owned by Roche Molecular Systems,
Inc., and F. Hoffman-LaRoche, Ltd.
