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> Nucleic Acid Prep &
Purification > Plasmid
DNA Purification > Promega MagneSil
Tfx*
Biomek® FX
Promega's Wizard MagneSil Tfx* System on the Biomek® FX
Promega Corporation – www.promega.com
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The Wizard MagneSil Tfx* System provides a simple and reliable method for the
rapid isolation of plasmid DNA in a multiwell format. The purified plasmid can be
used directly for transfection, as well as for other standard molecular biology techniques.
The use of MagneSil* Paramagnetic Particles(a) for lysate clearing as well
as DNA capture circumvents the need for centrifugation or vacuum manifolds,
allowing DNA purification with the Wizard MagneSil Tfx* System to be completely
automated. DNA purified with the Wizard MagneSil Tfx* System is low in common plasmid prep impurities. Chemical contaminants as well as RNA, protein, and endotoxin are greatly reduced to provide high-quality plasmid DNA suitable for transfection. The amount of DNA used in transfections will vary depending on the transfection reagent and the cell line used and should be optimized whenever a new transfection reagent or cell line is examined. The protocol herein has been developed for use on a Biomek® FX robotic workstation. The procedure requires no manual interventions and takes approximately 45 minutes to process a single 96-well plate. The protocol can be adapted to other robotic workstations. For information on an automated protocol for your system go to: www.promega.com/automethods/ and provide your contact information. An Automation Support Team member will contact you regarding a protocol for use with your particular system. The Wizard MagneSil Tfx* System uses alkaline-SDS lysis to generate the bacterial lysate and incorporates MagneSil* Paramagnetic Particles for both lysate clearing and plasmid purification. The procedure is performed using a number of simple steps:  |
* All trademarks are the property of their respective owners. Where applicable,
the PCR process is covered by patents owned by Roche Molecular Systems,
Inc., and F. Hoffman-LaRoche, Ltd.
