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> Nucleic Acid Prep &
Purification > Plasmid
DNA Purification > Promega Cells to Gels
MagneSil*
Biomek® FX
Promega's Cells to Gels MagneSil* Plasmid Purification Protocol
on the Biomek® FX
Promega Corporation – www.promega.com
Product Components
| Product | Size | Cat.# |
| Wizard* MagneSil* Plasmid Purification System | 4 × 96 | A1630 |
| 8 × 96 | A1631 | |
| Wizard* MagneSil* Plasmid Purification System, HTP1 | 100 × 96 | A1635 |
Cat.# A1630 includes sufficient reagents for a minimum of 4 × 96 well plates. Includes:
• 50ml Cell Resuspension Solution
• 60ml Cell Lysis Solution
• 60ml Neutralization Solution
• 19ml MagneSil* BLUE
• 30ml MagneSil* RED
• 50ml Elution Buffer
• 16 Collection Plates
• 1 Protocol
Available Separately
| Product | Size | Cat.# |
| MagneSil* BLUE | 100ml | A2201 |
| MagneSil* RED | 100ml | A1641 |
| Cell Resuspension Solution | 500ml | A7114 |
| Cell Lysis Solution | 500ml | A7132 |
| Neutralization Solution | 500ml | A7132 |
| Elution Buffer | 500ml | A1655 |
Materials to Be Supplied by the User
• 96-well, U-bottom plates (Greiner Cat.# 650101)
• Innovative Microplate reagent reservoirs
(Innovative Microplate Cat.# S30014)
• V-bottom, deep-well culture plates (Fisher Cat.# 07-200-700, Costar Cat.# 3960)
• 80% ethanol
• 100% isopropanol
• 5M NaCl
Many types of reservoir and deep-well culture plates are available. However, the MagnaBot* 96 Magnetic Separation Device is specifically designed for use with Greiner U-bottom microplates. Optional lids are available for Innovative Microplate reservoirs (Innovative Microplate Cat.# A50014)
Instrument requirements for the Biomek® FX Workstation
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 1 |
Minimum: Biomek® FX Software version 2.1 | |
| Beckman Coulter, Inc. | 1 |
96-channel POD | |
| Beckman Coulter, Inc. | 10 |
Minimum number of Labware Positions by 1 POD | |
| Beckman Coulter, Inc. | 1 |
Tip Loader | |
| Beckman Coulter, Inc. | 717251 | 3 |
Biomek® AP96 P250 Tips, Nonsterile |
| Beckman Coulter, Inc. | 719363 | 1 |
Biomek® FX Disposable Tip Wash ALP |
| Beckman Coulter, Inc. | 719804 | 3 |
Biomek® FX Single Position ALP with Indented Sides |
| Beckman Coulter, Inc. | 719360 | 1 |
High Density 16-Position ALP |
| Beckman Coulter, Inc. | 719361 | 1 |
Biomek® FX Heating/Cooling ALP |
| Beckman Coulter, Inc. | 379410 | 1 |
Orbital Shaker |
| Beckman Coulter, Inc. | 719366 | 1 |
Biomek® FX Device Controller |
| Additional Intrument Requirements | |||
| 1 |
Circulating water bath capable of 65°C (for the Heating/Cooling ALP) |
||
| Promega Corporation | V8151 | 1 |
MagnaBot* 96 Magnetic Separation Device |
| Promega Corporation | V8381 | 1 |
MagnaBot* Spacer |
| Promega Corporation | Z3271 | 1 |
Heat Transfer Block |
Storage Conditions: Store all components at 22–25°C.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
