eLabNotebook > Nucleic Acid Prep & Purification > Genomic DNA Purification > Promega MagneSil*
ONE Fixed Yield Plant BiomekŪ FX


Promega's Automated MagneSil* ONE, Fixed Yield Plant Genomic DNA Purification Protocol on the BiomekŪ FX

Promega Corporation – www.promega.com

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Click here to download a printable PDF version of the following:

Problem Possible Cause Solution
Low Yield
  • Alcohol present in eluted material
  • Make sure all Wash Buffer is aspirated before elution.
  • Increase drying time on the Heat Transfer Block.
Low Yield
  • Insufficient amount of starting material
  • The optimal amount of starting
    material should be determined for each plant species. See note in Section III.A, Step 1, for guidelines for leaf tissue samples.
Green or Cloudy DNA
  • Too much starting material
  • Decrease the amount of starting material by 30% and re-isolate DNA.
  • Centrifuge the plate and transfer the supernatant.
Green or Cloudy DNA
  • Cold laboratory temperatures may cause reagent precipitation
  • Centrifuge the plate and trasfer the supernatant.
MagneSil* Particles Visible in Eluate
  • MagneSil* Particle carryover into eluted DNA
  • Allow more time and mixing on the magnet before removing eluted DNA.
  • Use the minimal amount of MagneSil* Particles necessary to obtain the desired yield.
  • Remove particles by centrifuging the plate and transferring the supernatant.
DNA Too Dilute
  • Too much elution
  • Decrease elution volume to a minimum of 50µl.
DNA Too Dilute
  • Insufficient amount of starting material
  • The optimal amount of starting material should be determined for each plant species.
Amplification Failure
  • DNA degrades upon storage
  • Use TE buffer for elution. Store DNA at –20°C in polypropylene plates or tubes.
Amplification Failure
  • Plant may contain phenolics
  • Add 10mg insoluable PVPP (polyvinylpolypyrrolidone) to plant material before lysis and grinding step.


* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.

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