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> Nucleic Acid Prep &
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DNA Purification > Promega MagneSil*
ONE Fixed Yield Plant BiomekŪ FX
Promega's Automated MagneSil* ONE, Fixed Yield Plant Genomic
DNA Purification Protocol on the BiomekŪ FX
Promega Corporation – www.promega.com
Materials to Be Supplied by the User:
| Source | Part No. | Quantity | Description |
| Promega Corporation | V3031 | 1 | Deep Well MagnaBot* 96 Magnetic Separation Device |
| Promega Corporation | Z3301 | 1 | 1/4 inch Foam Spacer |
| Promega Corporation | A9161 | 1 | Collection Plate |
| Marsh | AB 0787 | 1 | Deep Well Plates, 1.2ml, 96 round wells |
| Corning | 3960 | 1 | Deep Well Plate, 2.2ml, 96 square wells, V-bottom |
| Corning | 3960 | 2 | Optional for use on deck without tipwash station: 2 additional Deep Well Plates, 2.2ml, 96 square wells, V-bottom |
| Promega Corporation | Z3271 | 1 | Heat Transfer Block |
| Promega Corporation | P1193 | 1 | TE (pH 8.0) or Nuclease-Free Water |
| SPEX CertiPrep, Inc. | N/A | 1 | 96-well plant tissue grinder e.g., Geno/Grinder® 2000 |
| User Supplied | N/A | 1 | Geno/Grinder® beads |
| User Supplied | N/A | 1 | Foil sealing tape (3M Scotch brand aluminum foil tape 425: 3 inches × 60 yards) |
Biomek FX Equipment
| Source | Part No. | Quantity | Description |
| Beckman Coulter, Inc. | 267689 | 1 | Biomek FX Software |
| Beckman Coulter, Inc. | 719368 | 1 | 96-channel Pod |
| Beckman Coulter, Inc. | 719357 | 15 | Minimum number of Labware positions by 1 Pod |
| Beckman Coulter, Inc. | 719356 | 1 | Tip Loader ALP |
| Beckman Coulter, Inc. | 719316 | 1 | Heating/Cooling ALP, Single Position |
| Beckman Coulter, Inc. | 710363 | 1 | Tip Wash Station |
| VWR International | 13272-200 | 1 | Recirculating Water Bath |
Consumables
| Source | Part No. | Quantity | Description |
| Promega Corporation | V3031 | 1 | Deep Well MagnaBot* 96 Magnetic Separation Device |
| Promega Corporation | Z3301 | 1 | 1/4 in Foam Spacer |
| Corning, Inc. | 3960 | 1 to 3 | Deep Well Plate, 2.2ml (or comparable) |
| Marsh | AB-0787 | 3 | Deep Well plate, 1.2ml (or comparable) |
| Promega Corporation | A9161 | 1 | Polystyrene U-bottom multiwell |
| Beckman Coulter, Inc. | 717251 | 4 | Biomek® AP96 P250 tips (rack) |
* All trademarks are the property of their respective owners. Where applicable,
the PCR process is covered by patents owned by Roche Molecular Systems,
Inc., and F. Hoffman-LaRoche, Ltd.
