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Promega's Automated Wizard* SV 96 PCR* Clean-Up System on
the Biomek® FX
Promega Corporation – www.promega.com
Product Components
| Product | Size | Cat.# |
| Wizard* SV 96 PCR Clean-Up System | 1 × 96 isolations | A9340 |
For Laboratory Use. Each system contains sufficient reagents for 96 isolations. Includes:
• 20ml Membrane Binding Solution
• 1 Binding Plate
• 25ml Nuclease-Free Water
• 1 DNA Elution Plate
• 1 Protocol
| Product | Size | Cat.# |
| Wizard* SV 96 PCR Clean-Up System | 4 × 96 isolations | A9341 |
For Laboratory Use. Each system contains sufficient reagents for 4 × 96 isolations. Includes:
• 100ml Membrane Binding Solution
• 4 Binding Plates
• 150ml Nuclease-Free Water
• 4 Collection Plates
• 1 Protocol
| Product | Size | Cat.# |
| Wizard* SV 96 PCR Clean-Up System | 8 × 96 isolations | A9342 |
For Laboratory Use. Each system contains sufficient reagents for 8 × 96 isolations. Includes:
• 2 × 100ml Membrane Binding Solution
• 8 Binding Plates
• 2 × 150ml Nuclease-Free Water
• 8 Collection Plates
• 1 Protocol
Storage Conditions: Store all components at room temperature (22–25°C). No
refrigeration is required. Keep Membrane Binding Solution protected from light. See
system label for expiration date.
Automated Processing Requirements for the Biomek® FX
A. Instrumentation Requirements for the Biomek® FX
|
The following is a list of Beckman Coulter parts that are required for automation
of the SV 96 PCR Clean-Up System on a Beckman Biomek® FX liquid handling
workstation.
|
| Source |
Part No. |
Qty. |
Description |
| Beckman Coulter, Inc. |
717001 |
1 |
Coulter Biomek® FX workstation |
| Beckman Coulter, Inc. |
267689 |
1 |
Coulter Biomek® FX Software Version 2.1 or higher |
| Beckman Coulter, Inc. |
719368 |
1 |
Coulter 96-channel POD |
| Beckman Coulter, Inc. |
719357 |
10 |
Coulter labware positions/POD (minimum of 10 required) |
| Beckman Coulter, Inc. |
719356 |
1 |
Coulter Tip loader |
| Beckman Coulter, Inc. |
719358 or 719360 |
1 |
Coulter SPE ALP |
| Beckman Coulter, Inc. |
Contact Beckman Coulter |
1 |
Coulter Holder ALP (for SPE) |
| Promega Corporation |
609005 |
1 |
Vacuum Valve Unit |
| Promega Corporation |
609670 |
1 |
Vacuum Filtration Manifold Base |
| Promega Corporation |
390792 |
1 |
Elution Spacer |
| Promega Corporation |
609597 |
1 |
36mm Vacuum Collar |
| Promega Corporation |
609676 |
1 |
Tubing Kit, Filtration System |
| Promega Corporation |
975796 |
1 |
Plastic Bottle, 4L |
| Promega Corporation |
975797 |
1 |
Cap |
Labware Requirements
| Source |
Part No. |
Qty. |
Description |
| Innovative Microplate |
S30014 |
2 |
Pyramid-bottom Reservoir Plates |
| Promega Corporation |
A9161 (4/pack) |
3 |
96-well Greiner U-bottom plates |
| Promega Corporation |
provided in SV 96 PCR Clean-Up System |
1 |
96-well Elution Plate |
| Promega Corporation |
provided by user |
1 |
non-skirted 96-well PCR plate |
| Promega Corporation |
V8261 |
1 |
Plate Stand |
| Promega Corporation |
V8251 |
1 |
PCR Clamp 96 |
| Promega Corporation |
provided in SV 96 PCR Clean-Up System |
1 |
SV 96 Binding Plate |
* All trademarks are the property of their respective owners. Where applicable,
the PCR process is covered by patents owned by Roche Molecular Systems,
Inc., and F. Hoffman-LaRoche, Ltd. eLabNotebook
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