eLabNotebook > Nucleic Acid Prep & Purification > PCR Cleanup > Promega Wizard* SV 96
Clean-up Biomek® FX


Promega's Automated Wizard* SV 96 PCR* Clean-Up System on the Biomek® FX

Promega Corporation – www.promega.com

Product Components
ProductSizeCat.#
Wizard* SV 96 PCR Clean-Up System1 × 96 isolationsA9340
For Laboratory Use. Each system contains sufficient reagents for 96 isolations. Includes:

• 20ml Membrane Binding Solution
• 1 Binding Plate
• 25ml Nuclease-Free Water
• 1 DNA Elution Plate
• 1 Protocol

ProductSizeCat.#
Wizard* SV 96 PCR Clean-Up System4 × 96 isolationsA9341
For Laboratory Use. Each system contains sufficient reagents for 4 × 96 isolations. Includes:

• 100ml Membrane Binding Solution
• 4 Binding Plates
• 150ml Nuclease-Free Water
• 4 Collection Plates
• 1 Protocol

ProductSizeCat.#
Wizard* SV 96 PCR Clean-Up System8 × 96 isolationsA9342
For Laboratory Use. Each system contains sufficient reagents for 8 × 96 isolations. Includes:

• 2 × 100ml Membrane Binding Solution
• 8 Binding Plates
• 2 × 150ml Nuclease-Free Water
• 8 Collection Plates
• 1 Protocol


Storage Conditions: Store all components at room temperature (22–25°C). No refrigeration is required. Keep Membrane Binding Solution protected from light. See system label for expiration date.



Automated Processing Requirements for the Biomek® FX

A. Instrumentation Requirements for the Biomek® FX

The following is a list of Beckman Coulter parts that are required for automation of the SV 96 PCR Clean-Up System on a Beckman Biomek® FX liquid handling workstation.

Source Part No. Qty. Description
Beckman Coulter, Inc. 717001
1
Coulter Biomek® FX workstation
Beckman Coulter, Inc. 267689
1
Coulter Biomek® FX Software Version 2.1 or higher
Beckman Coulter, Inc. 719368
1
Coulter 96-channel POD
Beckman Coulter, Inc. 719357
10
Coulter labware positions/POD (minimum of 10 required)
Beckman Coulter, Inc. 719356
1
Coulter Tip loader
Beckman Coulter, Inc. 719358 or 719360
1
Coulter SPE ALP
Beckman Coulter, Inc. Contact Beckman Coulter
1
Coulter Holder ALP (for SPE)
Promega Corporation 609005
1
Vacuum Valve Unit
Promega Corporation 609670
1
Vacuum Filtration Manifold Base
Promega Corporation 390792
1
Elution Spacer
Promega Corporation 609597
1
36mm Vacuum Collar
Promega Corporation 609676
1
Tubing Kit, Filtration System
Promega Corporation 975796
1
Plastic Bottle, 4L
Promega Corporation 975797
1
Cap




Labware Requirements
Source Part No. Qty. Description
Innovative Microplate S30014
2
Pyramid-bottom Reservoir Plates
Promega Corporation A9161 (4/pack)
3
96-well Greiner U-bottom plates
Promega Corporation provided in SV 96 PCR Clean-Up System
1
96-well Elution Plate
Promega Corporation provided by user
1
non-skirted 96-well PCR plate
Promega Corporation V8261
1
Plate Stand
Promega Corporation V8251
1
PCR Clamp 96
Promega Corporation provided in SV 96 PCR Clean-Up System
1
SV 96 Binding Plate



* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.

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