eLabNotebook > Nucleic Acid Prep & Purification > RNA Purification > Promega SV 96 Total RNA
Isolation Biomek® FX


Promega's Automated SV 96 Total RNA Isolation System on the Biomek® FX

Promega Corporation – www.promega.com

Product Components

Product
SV 96 Total RNA Isolation System 1 × 96 Z3500
For Laboratory Use. Each system contains sufficient reagents for 96 isolations. Includes:

• 50ml SV RNA Lysis Buffer
• 2ml ß-mercaptoethanol (48.7%)
• 1 vial DNase I (lyophilized)
• 750µl MnCl2, 0.09M
• 2.5ml Yellow Core Buffer
• 13.25ml SV DNase Stop Solution (concentrated)
• 58.8ml SV RNA Wash Solution (concentrated)
• 13ml Nuclease-Free Water
• 1 SV 96 Binding Plate
• 1 DNA Elution Plate* (wrapped)
• 3 Plate Sealers
• 1 Protocol

(*A second, unwrapped plate is used in the packaging to protect the nibs on the SV 96 Binding Plate. Do not use this unwrapped plate for elution.)

Product
SV 96 Total RNA Isolation System 5 × 96 Z3505
For Laboratory Use. Each system contains sufficient reagents for 5 × 96 isolations. Includes:

• 2 × 50ml SV RNA Lysis Buffer
• 2 × 2ml ß-mercaptoethanol (48.7%)
• 5 vials DNase I (lyophilized)
• 5 × 750µl MnCl2, 0.09M
• 5 × 2.5ml Yellow Core Buffer
• 5 × 13.25ml SV DNase Stop Solution (concentrated)
• 5 × 58.8ml SV RNA Wash Solution (concentrated)
• 5 × 13ml Nuclease-Free Water
• 5 Binding Plates
• 5 DNA Elution Plates* (wrapped)
• 6 Plate Sealers
• 1 Protocol

(*A sixth, unwrapped plate is used in the packaging to protect the nibs on the SV 96 Binding Plates. Do not use this unwrapped plate for elution.)


B. Biomek® FX Workstation Requirements

1. Instrument requirements for the Biomek® FX Workstation

Source Part No. Quantity Description
Beckman Coulter, Inc.
267689
1
Minimum: Biomek® FX Software version 2.1
Beckman Coulter, Inc.
719368
1
96-channel POD
Beckman Coulter, Inc.
719357
10
Minimum number of Labware Positions by 1 POD
Beckman Coulter, Inc.
719356
1
Tip Loader
Beckman Coulter, Inc.
719858 or 719860
1
SPE ALP
Beckman Coulter, Inc.
Contact
Beckman Coulter
1
Holder ALP (for SPE)
Promega Corporation
609005
1
Vacuum Valve Unit
Promega Corporation
609670
1
Vacuum Filtration Manifold Base
Promega Corporation
390792
1
Elution Spacer
Promega Corporation
609597
1
36mm Vacuum Collar
Promega Corporation
609676
1
Tubing Kit, Filtration System
Promega Corporation
975796
1
Plastic Bottle, 4L
Promega Corporation
975797
1
Cap


2. Labware Requirements for the Biomek® FX

Source Part No. Quantity Description
Promega Corporation Innovative Microplate Part# S30014
2
Pyramid Bottom Reservoir Plates
Promega Corporation Promega Cat.# A9161 (4 pk)
3
96-well U-bottom Plates
Promega Corporation Provided in SV 96 Total RNA Isolation System
1
96-well Elution Plate
Promega Corporation Clear-well, 96-well flatbottom tissue culture plate provided by user
1
96-well cell plate
Promega Corporation Provided in SV 96 Total RNA Isolation System
1
SV96 Binding Plate

* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.

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