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Promega's Automated SV 96 Total RNA Isolation System on the Biomek®
FX
Promega Corporation – www.promega.com
Product Components
Product
SV 96 Total RNA Isolation System 1 × 96 Z3500
For Laboratory Use. Each system contains sufficient reagents for 96 isolations.
Includes:
• 50ml SV RNA Lysis Buffer
• 2ml ß-mercaptoethanol (48.7%)
• 1 vial DNase I (lyophilized)
• 750µl MnCl2, 0.09M
• 2.5ml Yellow Core Buffer
• 13.25ml SV DNase Stop Solution (concentrated)
• 58.8ml SV RNA Wash Solution (concentrated)
• 13ml Nuclease-Free Water
• 1 SV 96 Binding Plate
• 1 DNA Elution Plate* (wrapped)
• 3 Plate Sealers
• 1 Protocol
(*A second, unwrapped plate is used in the packaging to protect the nibs
on the SV 96 Binding Plate. Do not use this unwrapped plate for elution.)
Product
SV 96 Total RNA Isolation System 5 × 96 Z3505
For Laboratory Use. Each system contains sufficient reagents for 5 × 96
isolations. Includes:
• 2 × 50ml SV RNA Lysis Buffer
• 2 × 2ml ß-mercaptoethanol (48.7%)
• 5 vials DNase I (lyophilized)
• 5 × 750µl MnCl2, 0.09M
• 5 × 2.5ml Yellow Core Buffer
• 5 × 13.25ml SV DNase Stop Solution (concentrated)
• 5 × 58.8ml SV RNA Wash Solution (concentrated)
• 5 × 13ml Nuclease-Free Water
• 5 Binding Plates
• 5 DNA Elution Plates* (wrapped)
• 6 Plate Sealers
• 1 Protocol
(*A sixth, unwrapped plate is used in the packaging to protect the nibs
on the SV 96 Binding Plates. Do not use this unwrapped plate for elution.)
B. Biomek® FX Workstation Requirements
1. Instrument requirements for the Biomek® FX Workstation
| Source |
Part No. |
Quantity |
Description |
| Beckman Coulter, Inc. |
267689 |
1 |
Minimum: Biomek® FX Software version 2.1 |
| Beckman Coulter, Inc. |
719368 |
1 |
96-channel POD |
| Beckman Coulter, Inc. |
719357 |
10 |
Minimum number of Labware Positions by 1 POD |
| Beckman Coulter, Inc. |
719356 |
1 |
Tip Loader |
| Beckman Coulter, Inc. |
719858 or 719860
|
1 |
SPE ALP |
| Beckman Coulter, Inc. |
Contact
Beckman Coulter
|
1 |
Holder ALP (for SPE) |
| Promega Corporation |
609005 |
1 |
Vacuum Valve Unit |
| Promega Corporation |
609670 |
1 |
Vacuum Filtration Manifold Base |
| Promega Corporation |
390792 |
1 |
Elution Spacer |
| Promega Corporation |
609597 |
1 |
36mm Vacuum Collar |
| Promega Corporation |
609676 |
1 |
Tubing Kit, Filtration System |
| Promega Corporation |
975796 |
1 |
Plastic Bottle, 4L |
| Promega Corporation |
975797 |
1 |
Cap |
2. Labware Requirements for the Biomek® FX
| Source |
Part No. |
Quantity |
Description |
| Promega Corporation |
Innovative Microplate Part# S30014 |
2 |
Pyramid Bottom Reservoir Plates |
| Promega Corporation |
Promega Cat.# A9161 (4 pk) |
3 |
96-well U-bottom Plates |
| Promega Corporation |
Provided in SV 96 Total RNA Isolation System |
1 |
96-well Elution Plate |
| Promega Corporation |
Clear-well, 96-well flatbottom tissue culture plate provided by
user |
1 |
96-well cell plate |
| Promega Corporation |
Provided in SV 96 Total RNA Isolation System |
1 |
SV96 Binding Plate |
* All trademarks are the property of their respective owners. Where applicable,
the PCR process is covered by patents owned by Roche Molecular Systems,
Inc., and F. Hoffman-LaRoche, Ltd.
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