Extract-N-AMP Biomek® 2000
Customer Application (system support)
Automated Application of Sigma-Aldrich Extract-N-Amp* Blood PCR* Kit on the Biomek® 2000 Laboratory Workstation
Greg Schmidt, Derek Douglas, Carol Kreader
Sigma-Aldrich Corp.
Biotechnology Division
P.O. Box 14508, Saint Louis, MO 63178
www.sigmaaldrich.com
The Sigma Extract-N-Amp Blood PCR procedure utilizing the Biomek
2000 Laboratory Workstation enables users to rapidly extract and amplify human genomic DNA from up to 96 samples of whole blood with minimal user intervention. Briefly, 5µL
of whole blood is mixed with 10µL of Extraction Solution, and the mixture is incubated at room temperature for 5 minutes. After adding 90µL
of Neutralization Solution, the extract is ready for PCR. An aliquot of the neutralized extract is then combined with the Extract-N-Amp Blood PCR Ready Mix and user-provided PCR primers to amplify target DNA. The PCR master mix comes in two formulations:
Extract-N-Amp Blood PCR mix and REDExtract-N-Amp* Blood PCR mix.
The Extract-N-Amp Blood PCR Ready Mix is a 2X reaction mixture containing buffer, salts,
dNTPs, and Taq polymerase. It also contains TaqStart* antibody for hot start PCR to enhance specificity. The
REDExtract-N-Amp Blood PCR kit also contains a tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.
Features
- Rapid extraction of gDNA along with ready to use PCR mix enables user to go from 96 samples of whole blood to PCR in 25 minutes.
- No vacuum or centrifuge steps required.
- No expensive magnetic separation devices required.
- No need for any type of purification, organic extraction, centrifugation or alcohol precipitation.
- Hot Start antibody included for highly specific PCR amplification of genomic DNA.
- Extract storage at 4°C for up to 6 months.
* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-LaRoche, Ltd.
